Phylogenetic analysis of tmRNA genes within a bacterial subgroup reveals a specific structural signature.

Brice Felden 1, 2, * Christian Massire 3 Eric Westhof 3 John Atkins 1 Raymond Gesteland 1
* Auteur correspondant
1 Department of Human Genetics [Salt Lake City]
2 Laboratoire de Biochimie Pharmaceutique
3 Architecture et Réactivité de L'ARN
Abstract : Bacterial tmRNA mediates a trans-translation reaction, which permits the recycling of stalled ribosomes and probably also contributes to the regulated expression of a subset of genes. Its action results in the addition of a small number of C-terminal amino acids to protein whose synthesis had stalled and these constitute a proteolytic recognition tag for the degradation of these incompletely synthesized proteins. Previous work has identified pseudoknots and stem-loops that are widely conserved in divergent bacteria. In the present work an alignment of tmRNA gene sequences within 13 beta-proteobacteria reveals an additional sub-structure specific for this bacterial group. This sub-structure is in pseudoknot Pk2, and consists of one to two additional stem-loop(s) capped by stable GNRA tetraloop(s). Three-dimensional models of tmRNA pseudoknot 2 (Pk2) containing various topological versions of the additional sub-structure suggest that the sub-structures likely point away from the core of the RNA, containing both the tRNA and the mRNA domains. A putative tertiary interaction has also been identified.
Type de document :
Article dans une revue
Nucleic Acids Research, Oxford University Press, 2001, 29 (7), pp.1602-7. 〈10.1093/nar/29.7.1602〉
Liste complète des métadonnées

Littérature citée [35 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-00718211
Contributeur : Annick Lefevre <>
Soumis le : jeudi 19 juillet 2012 - 08:55:13
Dernière modification le : mercredi 16 mai 2018 - 11:24:04
Document(s) archivé(s) le : samedi 20 octobre 2012 - 02:20:57

Fichier

NAR_2001.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

U835 | BIOSIT | UR1-UFR-SVE | UNIV-RENNES

Citation

Brice Felden, Christian Massire, Eric Westhof, John Atkins, Raymond Gesteland. Phylogenetic analysis of tmRNA genes within a bacterial subgroup reveals a specific structural signature.. Nucleic Acids Research, Oxford University Press, 2001, 29 (7), pp.1602-7. 〈10.1093/nar/29.7.1602〉. 〈inserm-00718211〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

169

Téléchargements de fichiers

100

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD