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Journal articles
Two-piece tmRNA in cyanobacteria and its structural analysis.
Abstract : tmRNA acts to rescue stalled bacterial ribosomes while encoding a peptide tag added trans-translationally to the nascent peptide, targeting it for proteolysis. The permuted gene structure found in a group of cyanobacteria is shown to produce a two-piece mature tmRNA, as had been observed previously for the independently permuted gene of alpha-proteobacteria. The pieces have been mapped onto the gene sequence and aligned for the permuted cyanobacterial tmRNA sequences, including four novel sequences. Structural probing and base pair co-variations support a secondary structure model in which two pairings in the tRNA-like domain hold the two pieces together, and the coding piece bearing the tag reading frame additionally contains a single transient pseudoknot and three other stem-loops. This represents a dramatic reduction in pseudoknot number from the five present in one-piece cyanobacterial tmRNA.
Cited literature [25 references]
https://www.hal.inserm.fr/inserm-00718155
Contributor : Annick Lefevre Connect in order to contact the contributor
Submitted on : Monday, July 16, 2012 - 1:32:05 PM
Last modification on : Thursday, January 14, 2021 - 11:17:13 AM
Long-term archiving on: : Wednesday, October 17, 2012 - 2:36:05 AM
Contributor : Annick Lefevre Connect in order to contact the contributor
Submitted on : Monday, July 16, 2012 - 1:32:05 PM
Last modification on : Thursday, January 14, 2021 - 11:17:13 AM
Long-term archiving on: : Wednesday, October 17, 2012 - 2:36:05 AM
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