Pre-binding of small protein B to a stalled ribosome triggers trans-translation.

Abstract : To rescue stalled ribosomes, eubacteria employ a molecule, transfer messenger RNA (tmRNA), which functions both as a tRNA and as an mRNA. With the help of small protein B (SmpB), tmRNA restarts protein synthesis and adds by the trans-translation mechanism a peptide tag to the stalled protein to target it for destruction by cellular proteases. Here, the cellular location and expression of endogenous SmpB were monitored in vivo. We report that SmpB is associated with 70S ribosomes and not in the soluble fraction, independently of the presence of tmRNA. In vitro, SmpB that is pre-bound to a stalled ribosome can trigger initiation of trans-translation. Our results demonstrate the existence of a novel pathway for the entry of tmRNA to the ribosome and for the trans-transfer of a nascent peptide chain from peptidyl-tRNA to charged tmRNA.
Type de document :
Article dans une revue
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (25), pp.25978-85. 〈10.1074/jbc.M314086200〉
Liste complète des métadonnées

Littérature citée [26 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00715041
Contributeur : Annick Lefevre <>
Soumis le : vendredi 6 juillet 2012 - 11:26:32
Dernière modification le : mercredi 16 mai 2018 - 11:24:04
Document(s) archivé(s) le : jeudi 15 décembre 2016 - 21:10:53

Fichier

 Accès restreint
Fichier visible le : jamais

Connectez-vous pour demander l'accès au fichier

Identifiants

Collections

Citation

Marc Hallier, Natalia Ivanova, Armelle Rametti, Michael Pavlov, Måns Ehrenberg, et al.. Pre-binding of small protein B to a stalled ribosome triggers trans-translation.. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2004, 279 (25), pp.25978-85. 〈10.1074/jbc.M314086200〉. 〈inserm-00715041〉

Partager

Métriques

Consultations de la notice

86