Design of biomimetic cell-interactive substrates using hyaluronic Acid hydrogels with tunable mechanical properties.

Emilie Hachet 1 Hélène Van den Berghe 1 Eric Bayma 1 Marc Block 2 Rachel Auzély-Velty 1, *
* Auteur correspondant
1 CERMAV - Centre de recherches sur les macromolécules végétales
2 INSERM U823, équipe 1 (DySAD)
INSERM U823 - Institut d'oncologie/développement Albert Bonniot de Grenoble, DySAD - Dynamique des systèmes d'adhérence et différenciation
Abstract : Hyaluronic acid (HA) is a natural polysaccharide abundant in biological tissues with excellent potential for constructing synthetic extracellular matrix analogues. In this work, we established a simple and dependable approach to prepare hyaluronic acid-based hydrogels with controlled stiffness and cell recognition properties for use as cell-interactive substrates. This approach relied on a new procedure for the synthesis of methacrylate-modified HA macromers (HA-MA) and, on photorheometry allowing real time monitoring of gelation during photopolymerization. We showed in this way the ability to obtain gels that encompass the range of physiologically relevant elastic moduli while still maintaining the recognition properties of HA by specific cell surface receptors. These hydrogels were prepared from HA macromers having a degree of methacrylation <0.5, which allows to minimize compromising effects on the binding affinity of HA to its cell receptors due to high substitution on the one hand, and to achieve nearly 100% conversion of the methacrylate groups on the other. When the HA hydrogels were immobilized on glass substrates, it was observed that the attachment and the spreading of a variety of mammalian cells rely on CD44 and its coreceptor RHAMM. The attachment and spreading were also shown to be modulated by the elastic properties of the HA matrix. All together, these results highlight the biological potential of these HA hydrogel systems and the needs of controlling their chemical and physical properties for applications in cell culture and tissue engineering.
Keywords : Hyluronic acid hydrogels mechanotransduction CD44 RHAMM
Type de document :
Article dans une revue
Biomacromolecules, American Chemical Society, 2012, 13 (6), pp.1818-27. 〈10.1021/bm300324m〉
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http://www.hal.inserm.fr/inserm-00712208
Contributeur : Marc Block <>
Soumis le : mercredi 26 juin 2013 - 07:00:04
Dernière modification le : jeudi 11 janvier 2018 - 06:26:36
Document(s) archivé(s) le : vendredi 31 mars 2017 - 09:52:17

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Emilie Hachet, Hélène Van den Berghe, Eric Bayma, Marc Block, Rachel Auzély-Velty. Design of biomimetic cell-interactive substrates using hyaluronic Acid hydrogels with tunable mechanical properties.. Biomacromolecules, American Chemical Society, 2012, 13 (6), pp.1818-27. 〈10.1021/bm300324m〉. 〈inserm-00712208〉

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