T. Doetschman, H. Eistetter, M. Katz, W. Schmidt, and R. Kemler, The in vitro development of blastocyst-derived embryonic stem cell lines: Formation of visceral yolk sac, blood islands and myocardium, J Embryol Exp Morphol, vol.87, pp.27-45, 1985.

M. Li, L. Pevny, R. Lovell-badge, and A. Smith, Generation of purified neural precursors from embryonic stem cells by lineage selection, Current Biology, vol.8, issue.17, pp.971-974, 1998.
DOI : 10.1016/S0960-9822(98)70399-9

S. Okabe, K. Forsberg-nilsson, A. Spiro, M. Segal, and R. Mckay, Development of neuronal precursor cells and functional postmitotic neurons from embryonic stem cells in vitro, Mechanisms of Development, vol.59, issue.1, pp.89-102, 1996.
DOI : 10.1016/0925-4773(96)00572-2

S. Nishikawa, S. Nishikawa, M. Hirashima, N. Matsuyoshi, and H. Kodama, Progressive lineage analysis by cell sorting and culture identifies FLK1þ VEcadherinþ cells at a diverging point of endothelial and hemopoietic lineages, Development, vol.125, pp.1747-1757, 1998.

J. Yamashita, H. Itoh, M. Hirashima, M. Ogawa, and S. Nishikawa, Flk1- positive cells derived from embryonic stem cells serve as vascular progenitors, Nature, vol.408, issue.6808, pp.92-96, 2000.
DOI : 10.1038/35040568

S. Tada, T. Era, C. Furusawa, H. Sakurai, and S. Nishikawa, Characterization of mesendoderm: a diverging point of the definitive endoderm and mesoderm in embryonic stem cell differentiation culture, Development, vol.132, issue.19, pp.4363-4374, 2005.
DOI : 10.1242/dev.02005

Q. Ying, M. Stavridis, D. Griffiths, M. Li, and A. Smith, Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture, Nature Biotechnology, vol.128, issue.2, pp.183-186, 2003.
DOI : 10.1038/nbt780

M. Stavridis and A. Smith, Neural differentiation of mouse embryonic stem cells, Biochemical Society Transactions, vol.31, issue.1, pp.45-49, 2003.
DOI : 10.1042/bst0310045

E. Lai, Notch signaling: control of cell communication and cell fate, Development, vol.131, issue.5, pp.965-973, 2004.
DOI : 10.1242/dev.01074

P. Heitzler and P. Simpson, The choice of cell fate in the epidermis of Drosophila, Cell, vol.64, issue.6, pp.1083-1092, 1991.
DOI : 10.1016/0092-8674(91)90263-X

N. Daudet and J. Lewis, Two contrasting roles for Notch activity in chick inner ear development: specification of prosensory patches and lateral inhibition of hair-cell differentiation, Development, vol.132, issue.3, pp.541-551, 2005.
DOI : 10.1242/dev.01589

P. Mountford, B. Zevnik, A. Duwel, J. Nichols, and M. Li, Dicistronic targeting constructs: reporters and modifiers of mammalian gene expression., Proceedings of the National Academy of Sciences, vol.91, issue.10, pp.4303-4307, 1994.
DOI : 10.1073/pnas.91.10.4303

Q. Ying, J. Nichols, E. Evans, and A. Smith, Changing potency by spontaneous fusion, Nature, vol.416, issue.6880, pp.545-548, 2002.
DOI : 10.1038/nature729
URL : https://www.era.lib.ed.ac.uk/bitstream/1842/707/2/Nichols_J.pdf

R. Kopan, J. Nye, and H. Weintraub, The intracellular domain of mouse Notch: A constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD, Development, vol.120, pp.2385-2396, 1994.

H. Niwa, S. Masui, I. Chambers, A. Smith, and J. Miyazaki, Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells, Molecular and Cellular Biology, vol.22, issue.5, pp.1526-1536, 2002.
DOI : 10.1128/MCB.22.5.1526-1536.2002

B. Zambrowicz, A. Imamoto, S. Fiering, L. Herzenberg, and W. Kerr, Disruption of overlapping transcripts in the ROSA ??geo 26 gene trap strain leads to widespread expression of ??-galactosidase in mouse embryos and hematopoietic cells, Proceedings of the National Academy of Sciences, vol.94, issue.8, pp.3789-3794, 1997.
DOI : 10.1073/pnas.94.8.3789

J. Aubert, H. Dunstan, I. Chambers, and A. Smith, Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation, Nature Biotechnology, vol.20, issue.12, pp.1240-1245, 2002.
DOI : 10.1038/nbt763

A. Smith, J. Heath, D. Donaldson, G. Wong, and J. Moreau, Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides, Nature, vol.336, issue.6200, pp.688-690, 1988.
DOI : 10.1038/336688a0

Q. Ying, J. Nichols, I. Chambers, and A. Smith, BMP Induction of Id Proteins Suppresses Differentiation and Sustains Embryonic Stem Cell Self-Renewal in Collaboration with STAT3, Cell, vol.115, issue.3, pp.281-292, 2003.
DOI : 10.1016/S0092-8674(03)00847-X

I. Chambers and A. Smith, Self-renewal of teratocarcinoma and embryonic stem cells, Oncogene, vol.23, issue.43, pp.7150-7160, 2004.
DOI : 10.1038/sj.onc.1207930

Q. Ying and A. Smith, Defined Conditions for Neural Commitment and Differentiation, Methods Enzymol, vol.365, pp.327-341, 2003.
DOI : 10.1016/S0076-6879(03)65023-8

H. Kawasaki, K. Mizuseki, S. Nishikawa, S. Kaneko, and Y. Kuwana, Induction of Midbrain Dopaminergic Neurons from ES Cells by Stromal Cell???Derived Inducing Activity, Neuron, vol.28, issue.1, pp.31-40, 2000.
DOI : 10.1016/S0896-6273(00)00083-0

H. Niwa, J. Miyazaki, and A. Smith, Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells, Nature Genetics, vol.24, issue.4, pp.372-376, 2000.
DOI : 10.1038/74199

J. Rathjen, J. Lake, M. Bettess, J. Washington, and G. Chapman, Formation of a primitive ectoderm like cell population, EPL cells, from ES cells in response to biologically derived factors, J Cell Sci, vol.112, pp.601-612, 1999.

J. Hebert, M. Boyle, and G. Martin, mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation, Development, vol.112, pp.407-415, 1991.

M. Shen and P. Leder, Leukemia inhibitory factor is expressed by the preimplantation uterus and selectively blocks primitive ectoderm formation in vitro., Proceedings of the National Academy of Sciences, vol.89, issue.17, pp.8240-8244, 1992.
DOI : 10.1073/pnas.89.17.8240

L. Vallier, J. Mancip, S. Markossian, A. Lukaszewicz, and C. Dehay, An efficient system for conditional gene expression in embryonic stem cells and in their in vitro and in vivo differentiated derivatives, Proceedings of the National Academy of Sciences, vol.98, issue.5, pp.2467-2472, 2001.
DOI : 10.1073/pnas.041617198
URL : https://hal.archives-ouvertes.fr/hal-00023806

H. Karlstrom, A. Bergman, U. Lendahl, J. Naslund, and J. Lundkvist, A Sensitive and Quantitative Assay for Measuring Cleavage of Presenilin Substrates, Journal of Biological Chemistry, vol.277, issue.9, pp.6763-6766, 2002.
DOI : 10.1074/jbc.C100649200

J. Ebinu and B. Yankner, A RIP Tide in Neuronal Signal Transduction, Neuron, vol.34, issue.4, pp.499-502, 2002.
DOI : 10.1016/S0896-6273(02)00704-3

G. Bain, W. Ray, M. Yao, and D. Gottlieb, Retinoic Acid Promotes Neural and Represses Mesodermal Gene Expression in Mouse Embryonic Stem Cells in Culture, Biochemical and Biophysical Research Communications, vol.223, issue.3, pp.691-694, 1996.
DOI : 10.1006/bbrc.1996.0957

T. Schroeder, S. Fraser, M. Ogawa, S. Nishikawa, and C. Oka, Recombination signal sequence-binding protein J?? alters mesodermal cell fate decisions by suppressing cardiomyogenesis, Proceedings of the National Academy of Sciences, vol.100, issue.7, pp.4018-4023, 2003.
DOI : 10.1073/pnas.0438008100

S. Hitoshi, T. Alexson, V. Tropepe, D. Donoviel, and A. Elia, Notch pathway molecules are essential for the maintenance, but not the generation, of mammalian neural stem cells, Genes & Development, vol.16, issue.7, pp.846-858, 2002.
DOI : 10.1101/gad.975202

P. Ellis, B. Fagan, S. Magness, S. Hutton, and O. Taranova, SOX2, a Persistent Marker for Multipotential Neural Stem Cells Derived from Embryonic Stem Cells, the Embryo or the Adult, Developmental Neuroscience, vol.26, issue.2-4, pp.148-165, 2004.
DOI : 10.1159/000082134

N. Lumelsky, O. Blondel, P. Laeng, I. Velasco, and R. Ravin, Differentiation of Embryonic Stem Cells to Insulin-Secreting Structures Similar to Pancreatic Islets, Science, vol.292, issue.5520, pp.1389-1394, 2001.
DOI : 10.1126/science.1058866

T. Sejersen and U. Lendahl, Transient expression of the intermediate filament nestin during skeletal muscle development, J Cell Sci, vol.106, pp.1291-1300, 1993.

A. Streit, A. Berliner, C. Papanayotou, A. Sirulnik, and C. Stern, Initiation of neural induction by FGF signalling before gastrulation, Nature, vol.406, issue.6791, pp.74-78, 2000.
DOI : 10.1038/35017617

S. Wilson, E. Graziano, H. R. Jessell, T. Edlund, and T. , An early requirement for FGF signalling in the acquisition of neural cell fate in the chick embryo, Current Biology, vol.10, issue.8, pp.421-429, 2000.
DOI : 10.1016/S0960-9822(00)00431-0

E. Pera, A. Ikeda, E. Eivers, D. Robertis, and E. , Integration of IGF, FGF, and anti-BMP signals via Smad1 phosphorylation in neural induction, Genes & Development, vol.17, issue.24, pp.3023-3028, 2003.
DOI : 10.1101/gad.1153603

M. Mohammadi, G. Mcmahon, L. Sun, C. Tang, and P. Hirth, Structures of the Tyrosine Kinase Domain of Fibroblast Growth Factor Receptor in Complex with Inhibitors, Science, vol.276, issue.5314, pp.955-960, 1997.
DOI : 10.1126/science.276.5314.955

Y. Dai, C. Yu, V. Singh, L. Tang, and Z. Wang, Pharmacological inhibitors of the mitogen-activated protein kinase (MAPK) kinase/MAPK cascade interact synergistically with UCN-01 to induce mitochondrial dysfunction and apoptosis in human leukemia cells, Cancer Res, vol.61, pp.5106-5115, 2001.

M. Gotz and Y. Barde, Radial Glial Cells, Neuron, vol.46, issue.3, pp.369-372, 2005.
DOI : 10.1016/j.neuron.2005.04.012

L. Conti, S. Pollard, T. Gorba, E. Reitano, and M. Toselli, Nicheindependent symmetrical self-renewal of a mammalian tissue stem cell, PLoS Biol, vol.3, 2005.

T. Ohtsuka, M. Sakamoto, F. Guillemot, and R. Kageyama, Roles of the Basic Helix-Loop-Helix Genes Hes1 and Hes5 in Expansion of Neural Stem Cells of the Developing Brain, Journal of Biological Chemistry, vol.276, issue.32, pp.30467-30474, 2001.
DOI : 10.1074/jbc.M102420200

K. Tanigaki, F. Nogaki, J. Takahashi, K. Tashiro, and H. Kurooka, Notch1 and Notch3 Instructively Restrict bFGF-Responsive Multipotent Neural Progenitor Cells to an Astroglial Fate, Neuron, vol.29, issue.1, pp.45-55, 2001.
DOI : 10.1016/S0896-6273(01)00179-9
URL : http://doi.org/10.1016/s0896-6273(01)00179-9

J. Walsh and P. Andrews, Expression of Wnt and Notch pathway genes in a pluripotent human embryonal carcinoma cell line and embryonic stem cells, APMIS, vol.79, issue.1, pp.197-210, 2003.
DOI : 10.1006/geno.1996.0009

J. Rho, K. Yu, J. Han, J. Chae, and D. Koo, Transcriptional profiling of the developmentally important signalling pathways in human embryonic stem cells, Human Reproduction, vol.21, issue.2, pp.405-412, 2006.
DOI : 10.1093/humrep/dei328

S. Hoflinger, K. Kesavan, M. Fuxa, C. Hutter, and B. Heavey, Analysis of Notch1 Function by In Vitro T Cell Differentiation of Pax5 Mutant Lymphoid Progenitors, The Journal of Immunology, vol.173, issue.6, pp.3935-3944, 2004.
DOI : 10.4049/jimmunol.173.6.3935

S. Lowell, P. Jones, L. Roux, I. Dunne, J. Watt et al., Stimulation of human epidermal differentiation by Delta???Notch signalling at the boundaries of stem-cell clusters, Current Biology, vol.10, issue.9, pp.491-500, 2000.
DOI : 10.1016/S0960-9822(00)00451-6

T. Schmitt and J. Zuniga-pflucker, Induction of T Cell Development from Hematopoietic Progenitor Cells by Delta-like-1 In Vitro, Immunity, vol.17, issue.6, pp.749-756, 2002.
DOI : 10.1016/S1074-7613(02)00474-0

X. Li, Z. Du, E. Zarnowska, M. Pankratz, and L. Hansen, Specification of motoneurons from human embryonic stem cells, Nature Biotechnology, vol.129, issue.2, pp.215-221, 2005.
DOI : 10.1016/j.ydbio.2003.12.034

A. Perrier, V. Tabar, T. Barberi, M. Rubio, and J. Bruses, Derivation of midbrain dopamine neurons from human embryonic stem cells, Proceedings of the National Academy of Sciences, vol.101, issue.34, pp.12543-12548, 2004.
DOI : 10.1073/pnas.0404700101

S. Morrison, S. Perez, Z. Qiao, J. Verdi, and C. Hicks, Transient Notch Activation Initiates an Irreversible Switch from Neurogenesis to Gliogenesis by Neural Crest Stem Cells, Cell, vol.101, issue.5, pp.499-510, 2000.
DOI : 10.1016/S0092-8674(00)80860-0

A. Smith, Converting ES Cell into Neurons, Stem Cells in the Nervous System: Functional and Clinical Implications, pp.83-94, 2004.
DOI : 10.1007/978-3-642-18883-1_6

M. Morimoto, Y. Takahashi, M. Endo, and Y. Saga, The Mesp2 transcription factor establishes segmental borders by suppressing Notch activity, Nature, vol.128, issue.7040, pp.354-359, 2005.
DOI : 10.1038/nbt0102-87

P. Rathjen, J. Nichols, S. Toth, D. Edwards, and J. Heath, Developmentally programmed induction of differentiation inhibiting activity and the control of stem cell populations., Genes & Development, vol.4, issue.12b, pp.2308-2318, 1990.
DOI : 10.1101/gad.4.12b.2308

S. Zhao, J. Nichols, A. Smith, and M. Li, SoxB transcription factors specify neuroectodermal lineage choice in ES cells, Molecular and Cellular Neuroscience, vol.27, issue.3, pp.332-342, 2004.
DOI : 10.1016/j.mcn.2004.08.002

N. Gaiano, J. Nye, and G. Fishell, Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain, Neuron, vol.26, issue.2, pp.395-404, 2000.
DOI : 10.1016/S0896-6273(00)81172-1

K. Yoon and N. Gaiano, Notch signaling in the mammalian central nervous system: insights from mouse mutants, Nature Neuroscience, vol.23, issue.6, pp.709-715, 2005.
DOI : 10.1126/SCIENCE.1065412

T. Anthony, H. Mason, T. Gridley, G. Fishell, and N. Heintz, Brain lipid-binding protein is a direct target of Notch signaling in radial glial cells, Genes & Development, vol.19, issue.9, pp.1028-1033, 2005.
DOI : 10.1101/gad.1302105

W. Ge, K. Martinowich, X. Wu, F. He, and A. Miyamoto, Notch signaling promotes astrogliogenesis via direct CSL-mediated glial gene activation, Journal of Neuroscience Research, vol.20, issue.6, pp.848-860, 2002.
DOI : 10.1002/jnr.10364

Y. Li and N. Baker, Proneural enhancement by Notch overcomes Suppressor-of-Hairless repressor function in the developing Drosophila eye, Current Biology, vol.11, issue.5, pp.330-338, 2001.
DOI : 10.1016/S0960-9822(01)00093-8

S. Wilson and T. Edlund, Neural induction: Toward a unifying mechanism, Nature Neuroscience, vol.4, issue.Supp, pp.1161-1168, 2001.
DOI : 10.1038/nn747

C. Linker and C. Stern, Neural induction requires BMP inhibition only as a late step, and involves signals other than FGF and Wnt antagonists, Development, vol.131, issue.22, pp.5671-5681, 2004.
DOI : 10.1242/dev.01445

J. De-la-pompa, A. Wakeham, K. Correia, E. Samper, and S. Brown, Conservation of the Notch signalling pathway in mammalian neurogenesis, Development, vol.124, pp.1139-1148, 1997.

C. Shawber, D. Nofziger, J. Hsieh, C. Lindsell, and O. Bogler, Notch signaling inhibits muscle cell differentiation through a CBF1-independent pathway, Development, vol.122, pp.3765-3773, 1996.

C. Oka, T. Nakano, A. Wakeham, J. De-la-pompa, and C. Mori, Disruption of the mouse RBP-J kappa gene results in early embryonic death, Development, vol.121, pp.3291-3301, 1995.

Y. Jiang, B. Aerne, L. Smithers, C. Haddon, and D. Ish-horowicz, Notch signalling and the synchronization of the somite segmentation clock, Nature, vol.408, pp.475-479, 2000.

H. Niwa, K. Yamamura, and J. Miyazaki, Efficient selection for highexpression transfectants with a novel eukaryotic vector, Gene, vol.108, pp.193-199, 1991.

S. Jarriault, C. Brou, F. Logeat, E. Schroeter, and R. Kopan, Signalling downstream of activated mammalian Notch, Nature, vol.377, issue.6547, pp.355-358, 1995.
DOI : 10.1038/377355a0

A. Smith, Culture and differentiation of embryonic stem cells, Journal of Tissue Culture Methods, vol.336, issue.2, pp.89-94, 1991.
DOI : 10.1007/BF01666137

O. Hovatta, M. Mikkola, K. Gertow, A. Stromberg, and J. Inzunza, A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells, Human Reproduction, vol.18, issue.7, pp.1404-1409, 2003.
DOI : 10.1093/humrep/deg290

H. Kataoka, N. Takakura, S. Nishikawa, K. Tsuchida, and H. Kodama, Expressions of PDGF receptor alpha, c-Kit and Flk1 genes clustering in mouse chromosome 5 define distinct subsets of nascent mesodermal cells, Development, Growth and Differentiation, vol.118, issue.6, pp.729-740, 1997.
DOI : 10.1111/1523-1747.ep12371802

N. Takakura, H. Yoshida, T. Kunisada, S. Nishikawa, and S. Nishikawa, Involvement of Platelet-Derived Growth Factor Receptor-?? in Hair Canal Formation, Journal of Investigative Dermatology, vol.107, issue.5, pp.770-777, 1996.
DOI : 10.1111/1523-1747.ep12371802

Y. Shirayoshi, A. Nose, K. Iwasaki, and M. Takeichi, N-linked oligosaccharides are not involved in the function of a cell-cell binding glycoprotein E-cadherin., Cell Structure and Function, vol.11, issue.3, pp.245-252, 1986.
DOI : 10.1247/csf.11.245