Skip to Main content Skip to Navigation
Journal articles

Characterization of sleep in zebrafish and insomnia in hypocretin receptor mutants.

Abstract : Sleep is a fundamental biological process conserved across the animal kingdom. The study of how sleep regulatory networks are conserved is needed to better understand sleep across evolution. We present a detailed description of a sleep state in adult zebrafish characterized by reversible periods of immobility, increased arousal threshold, and place preference. Rest deprivation using gentle electrical stimulation is followed by a sleep rebound, indicating homeostatic regulation. In contrast to mammals and similarly to birds, light suppresses sleep in zebrafish, with no evidence for a sleep rebound. We also identify a null mutation in the sole receptor for the wake-promoting neuropeptide hypocretin (orexin) in zebrafish. Fish lacking this receptor demonstrate short and fragmented sleep in the dark, in striking contrast to the excessive sleepiness and cataplexy of narcolepsy in mammals. Consistent with this observation, we find that the hypocretin receptor does not colocalize with known major wake-promoting monoaminergic and cholinergic cell groups in the zebrafish. Instead, it colocalizes with large populations of GABAergic neurons, including a subpopulation of Adra2a-positive GABAergic cells in the anterior hypothalamic area, neurons that could assume a sleep modulatory role. Our study validates the use of zebrafish for the study of sleep and indicates molecular diversity in sleep regulatory networks across vertebrates.
Document type :
Journal articles
Complete list of metadatas

Cited literature [87 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00707849
Contributor : Delphine Autard <>
Submitted on : Wednesday, June 13, 2012 - 4:08:02 PM
Last modification on : Saturday, September 19, 2020 - 4:46:24 AM
Long-term archiving on: : Friday, September 14, 2012 - 2:36:49 AM

File

journal.pbio.0050277.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Tohei Yokogawa, Wilfredo Marin, Juliette Faraco, Guillaume Pézeron, Lior Appelbaum, et al.. Characterization of sleep in zebrafish and insomnia in hypocretin receptor mutants.. PLoS Biology, Public Library of Science, 2007, 5 (10), pp.e277. ⟨10.1371/journal.pbio.0050277⟩. ⟨inserm-00707849⟩

Share

Metrics

Record views

310

Files downloads

569