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Journal Articles Retrovirology Year : 2012

Host-pathogen interactome mapping for HTLV-1 and 2 retroviruses.

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Irma Lemmens
  • Function : Author
  • PersonId : 925138
Jean-Stéphane Gatot
  • Function : Author
  • PersonId : 925141
Didier Vertommen
  • Function : Author
  • PersonId : 925144
Vincent Navratil
  • Function : Author
  • PersonId : 906068
Carine van Lint
  • Function : Author
  • PersonId : 925153
Jan Tavernier
  • Function : Author
  • PersonId : 869890

Abstract

ABSTRACT: BACKGROUND: Human T-cell leukemia virus type 1 (HTLV-1) and type 2 both target T lymphocytes, yet induce radically different phenotypic outcomes. HTLV-1 is a causative agent of Adult T-cell leukemia (ATL), whereas HTLV-2, highly similar to HTLV-1, causes no known overt disease. HTLV gene products are engaged in a dynamic struggle of activating and antagonistic interactions with host cells. Investigations focused on one or a few genes have identified several human factors interacting with HTLV viral proteins. Most of the available interaction data concern the highly investigated HTLV-1 Tax protein. Identifying shared and distinct host-pathogen protein interaction profiles for these two viruses would enlighten how they exploit distinctive or common strategies to subvert cellular pathways toward disease progression. RESULTS: We employ a scalable methodology for the systematic mapping and comparison of pathogen-host protein interactions that includes stringent yeast two-hybrid screening and systematic retest, as well as two independent validations through an additional protein interaction detection method and a functional transactivation assay. The final data set contained 166 interactions between 10 viral proteins and 122 human proteins. Among the 166 interactions identified, 87 and 79 involved HTLV-1 and HTLV-2 -encoded proteins, respectively. Targets for HTLV-1 and HTLV-2 proteins implicate a diverse set of cellular processes including the ubiquitin-proteasome system, the apoptosis, different cancer pathways and the Notch signaling pathway. CONCLUSIONS: This study constitutes a first pass, with homogeneous data, at comparative analysis of host targets for HTLV-1 and 2 retroviruses, complements currently existing data for formulation of systems biology models of retroviral induced diseases and presents new insights on biological pathways involved in retroviral infection.
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Dates and versions

inserm-00697435 , version 1 (15-05-2012)

Licence

Attribution - CC BY 4.0

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Cite

Nicolas Simonis, Jean-François Rual, Irma Lemmens, Mathieu Boxus, Tomoko Hirozane-Kishikawa, et al.. Host-pathogen interactome mapping for HTLV-1 and 2 retroviruses.. Retrovirology, 2012, 9 (1), pp.26. ⟨10.1186/1742-4690-9-26⟩. ⟨inserm-00697435⟩

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