Human adipose tissue macrophages display activation of cancer-related pathways.: ATM link obesity and cancer?

Abstract : Obesity is associated with a significantly increased risk for cancer suggesting that adipose tissue dysfunctions might play a crucial role therein. Macrophages play important roles in adipose tissue as well as in cancers. Here, we studied whether human adipose tissue macrophages (ATM) modulate cancer cell function. Therefore, ATM were isolated and compared with monocyte-derived macrophages (MDM) from the same obese patients. ATM, but not MDM, were found to secrete factors inducing inflammation and lipid accumulation in human T47D and HT-29 cancer cells. Gene expression profile comparison of ATM and MDM revealed overexpression of functional clusters, such as cytokine-cytokine receptor interaction (especially CXC-chemokine) signaling as well as cancer-related pathways, in ATM. Comparison with gene expression profiles of human tumor-associated macrophages showed that ATM, but not MDM resemble tumor-associated macrophages. Indirect co-culture experiments demonstrated that factors secreted by preadipocytes, but not mature adipocytes, confer an ATM-like phenotype to MDM. Finally, the concentrations of ATM-secreted factors related to cancer are elevated in serum of obese subjects. In conclusion, ATM may thus modulate the cancer cell phenotype.
Type de document :
Article dans une revue
Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (26), pp.21904-13. 〈10.1074/jbc.M111.315200〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00690847
Contributeur : Marie-Hélène Derudas <>
Soumis le : mercredi 24 avril 2013 - 07:00:05
Dernière modification le : lundi 8 octobre 2018 - 17:44:07
Document(s) archivé(s) le : jeudi 25 juillet 2013 - 02:20:09

Identifiants

Collections

Citation

Therese Hervee Mayi, Mehdi Daoudi, Bruno Derudas, Barbara Gross, Gael Bories, et al.. Human adipose tissue macrophages display activation of cancer-related pathways.: ATM link obesity and cancer?. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2012, 287 (26), pp.21904-13. 〈10.1074/jbc.M111.315200〉. 〈inserm-00690847〉

Partager

Métriques

Consultations de la notice

425

Téléchargements de fichiers

897