Skip to Main content Skip to Navigation
Journal articles

Mnk1 kinase activity is required for abscission.

Abstract : Mnk1 is a serine/threonine kinase identified as a target for MAP kinase pathways. Using chemical drug, kinase-dead expression or knock down by RNA interference, we show that inhibition of Mnk1 induces the formation of multinucleated cells, which can be rescued by expressing an RNA interference resistant form of Mnk1. We found that active human Mnk1 localises to centrosomes, spindle microtubules and the midbody. Time-lapse recording of Mnk1 depleted cells display cytokinesis defects, as daughter cells fuse back together. Under inhibition of Mnk1 activity, no microtubule defect at the midbody was detected, however membrane vesicles anchorage at the midbody was impaired as lumenal-GFP positive-vesicles did not accumulate at the midbody. At the molecular level, we found that centriolin localisation was impaired at the midbody in Mnk1 depleted cells. As a consequence endobrevin, a V-SNARE protein implicated in the abscission step, was not properly localised at the midbody. Altogether our data show that Mnk1 activity is required for abscission.
Document type :
Journal articles
Complete list of metadatas

Cited literature [39 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00684686
Contributor : Hervé de Villemeur <>
Submitted on : Tuesday, October 8, 2013 - 2:50:36 PM
Last modification on : Wednesday, May 16, 2018 - 11:23:52 AM
Long-term archiving on: : Thursday, January 9, 2014 - 2:25:13 AM

File

MNK1_kinase_activity.accepted....
Publisher files allowed on an open archive

Identifiers

Citation

Yoann Rannou, Patrick Salaun, Christelle Benaud, Jabbar Khan, Stéphanie Dutertre, et al.. Mnk1 kinase activity is required for abscission.. Journal of Cell Science, Company of Biologists, 2012, 125 (Pt 12), pp.2844-52. ⟨10.1242/jcs.058081⟩. ⟨inserm-00684686⟩

Share

Metrics

Record views

556

Files downloads

443