Aurora kinase-C-T191D is constitutively active mutant.

Abstract : ABSTRACT: BACKGROUND: Aurora kinases (Aurora-A, B and C) belong to a family of conserved serine/threonine kinases which are key regulators of cell cycle progression. Aurora-A and Aurora-B are expressed in somatic cells and involved in cell cycle regulation while aurora-C is meiotic chromosome passenger protein. As Aurora kinase C is rarely expressed in normal somatic cells and has been found over expressed in many cancer lines. It is suggested that Aurora-C-T191D is not hyperactive mutant. Result Aurora-C-T191D variant form was investigated and compared with wild type. The overexpression of Aurora-C-T191D was observed that it behaves like Aurora-C wild type (aurC-WT). Both Aurora-C-T191D and aurC-WT induce abnormal cell division resulting in centrosome amplification and multinucleation in transiently transfected cells as well as in stable cell lines. Similarly, Aurora-C-T191D and aurC-WT formed foci of colonies when grown on soft agar, indicating that a gain of Aurora-C activity is sufficient to transform cells. Furthermore, we reported that NIH-3 T3 stable cell lines overexpressing Aurora-C-T191D and its wild type partner induced tumour formation when injected into nude mice, demonstrating the oncogenic activity of enzymatically active Aurora kinase C. Interestingly enough tumour aggressiveness was positively correlated with the rate of kinase activity, making Aurora-C a potential anti-cancer therapeutic target. CONCLUSION: These findings proved that Aurora C-T191D is not hyperactive but is constitutively active mutant.
Type de document :
Article dans une revue
BMC Cell Biology, BioMed Central, 2012, 13 (1), pp.8. 〈10.1186/1471-2121-13-8〉
Liste complète des métadonnées

Littérature citée [26 références]  Voir  Masquer  Télécharger
Contributeur : Hervé De Villemeur <>
Soumis le : jeudi 29 mars 2012 - 11:52:09
Dernière modification le : mercredi 16 mai 2018 - 11:23:52
Document(s) archivé(s) le : samedi 30 juin 2012 - 02:26:04


Fichiers éditeurs autorisés sur une archive ouverte




Jabbar Khan, Sanaullah Khan, Sobia Attaullah, Ijaz Ali, Shahid Niaz Khan. Aurora kinase-C-T191D is constitutively active mutant.. BMC Cell Biology, BioMed Central, 2012, 13 (1), pp.8. 〈10.1186/1471-2121-13-8〉. 〈inserm-00683588〉



Consultations de la notice


Téléchargements de fichiers