Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle.

Abstract : ABSTRACT: BACKGROUND: The clonal transmission of lineage-specific DNA methylation patterns in a mammalian genome during the cellular division is a crucial biological process controlled by the DNA methyltransferase Dnmt1, mainly. To investigate possible dynamic mechanisms of DNA methylation inheritance during the cell cycle, we used a Proximity Ligation In Situ Assay (P-LISA) to analyze the kinetic of formation and DNA recruitment of Dnmt1-including complexes. RESULTS: P-LISA, sequential chromatin immunoprecipitation and quantitative methylation specific PCR revealed that the Dnmt1/PCNA/UHRF1-including complexes are mainly formed and recruited on DNA during the S-phase of cell cycle, while the formation and the DNA recruitment of several Dnmt1/transcription factors-including complexes are not S-phase dependent but are G0/G1 and/or G2/M phases dependent. CONCLUSION: Our data confirm that DNA methylation inheritance occurs in S-phase, and demonstrate that DNA methylation inheritance can also occur in G0/G1 and G2/M phases of the cell cycle.
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Article dans une revue
Cell Division, BioMed Central, 2012, 7 (1), pp.5. 〈10.1186/1747-1028-7-5〉
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Soumis le : lundi 19 mars 2012 - 21:10:08
Dernière modification le : jeudi 20 septembre 2018 - 17:02:01

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Eric Hervouet, Arulraj Nadaradjane, Marine Gueguen, François Vallette, Pierre-François Cartron. Kinetics of DNA methylation inheritance by the Dnmt1-including complexes during the cell cycle.. Cell Division, BioMed Central, 2012, 7 (1), pp.5. 〈10.1186/1747-1028-7-5〉. 〈inserm-00680687〉

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