Nucleophosmin/B23 activates Aurora A at the centrosome through phosphorylation of serine 89.: Activation of Aurora-A by Nucleophosmin

Abstract : Aurora A (AurA) is a major mitotic protein kinase involved in centrosome maturation and spindle assembly. Nucleophosmin/B23 (NPM) is a pleiotropic nucleolar protein involved in a variety of cellular processes including centrosome maturation. In the present study, we report that NPM is a strong activator of AurA kinase activity. NPM and AurA coimmunoprecipitate and colocalize to centrosomes in G2 phase, where AurA becomes active. In contrast with previously characterized AurA activators, NPM does not trigger autophosphorylation of AurA on threonine 288. NPM induces phosphorylation of AurA on serine 89, and this phosphorylation is necessary for activation of AurA. These data were confirmed in vivo, as depletion of NPM by ribonucleic acid interference eliminated phosphorylation of CDC25B on S353 at the centrosome, indicating a local loss of AurA activity. Our data demonstrate that NPM is a strong activator of AurA kinase activity at the centrosome and support a novel mechanism of activation for AurA.
Type de document :
Article dans une revue
Journal of Cell Biology, Rockefeller University Press, 2012, 197 (1), pp.19-26. 〈10.1083/jcb.201107134〉
Liste complète des métadonnées

Littérature citée [34 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00679670
Contributeur : Hervé De Villemeur <>
Soumis le : mercredi 28 mars 2012 - 14:20:07
Dernière modification le : mercredi 16 mai 2018 - 11:23:52

Fichier

Reboutier_JCB_2012_RUP.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

David Reboutier, Marie-Bérengère Troadec, Jean-Yves Cremet, Kenji Fukasawa, Claude Prigent. Nucleophosmin/B23 activates Aurora A at the centrosome through phosphorylation of serine 89.: Activation of Aurora-A by Nucleophosmin. Journal of Cell Biology, Rockefeller University Press, 2012, 197 (1), pp.19-26. 〈10.1083/jcb.201107134〉. 〈inserm-00679670v2〉

Partager

Métriques

Consultations de la notice

220

Téléchargements de fichiers

142