Expression and localization of claudins-3 and -12 in transformed human brain endothelium. - Archive ouverte HAL Access content directly
Journal Articles Fluids and Barriers of the CNS Year : 2012

Expression and localization of claudins-3 and -12 in transformed human brain endothelium.

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Abstract

UNLABELLED: ABSTRACT: BACKGROUND: The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. FINDINGS: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. CONCLUSIONS: These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
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inserm-00678794 , version 1 (14-03-2012)

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Anja Schrade, Hadassah Sade, Pierre-Olivier Couraud, Ignacio Romero, Babette Weksler, et al.. Expression and localization of claudins-3 and -12 in transformed human brain endothelium.. Fluids and Barriers of the CNS, 2012, 9 (1), pp.6. ⟨10.1186/2045-8118-9-6⟩. ⟨inserm-00678794⟩
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