ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target.

Sandra-Nadia Bikeye 1 Carole Colin 2 Yannick Marie 1 Raphaël Vampouille 1 Philippe Ravassard 1 Audrey Rousseau 3 Blandine Boisselier 1 Ahmed Idbaih 1, 4 Charles Calvo 1 Pascal Leuraud 5 Myriam Lassalle 5 Soufiane El Hallani 1 Jean-Yves Delattre 1, 4 Marc Sanson 1, 4, *
* Auteur correspondant
1 CRICM - Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière
2 CRO2 - Centre de Recherches en Oncologie biologique et Oncopharmacologie
3 Service de neuro-pathologie
4 Service de neurologie 1 [CHU Pitié-Salpétrière]
5 XenTech
Abstract : BACKGROUND: ASPM (Abnormal Spindle-like Microcephaly associated) over-expression was recently implicated in the development of malignant gliomas. RESULTS: To better characterize the involvement of ASPM in gliomas, we investigated the mRNA expression in 175 samples, including 8 WHO Grade II, 75 WHO Grade III and 92 WHO Grade IV tumors. Aspm expression was strongly correlated with tumor grade and increased at recurrence when compared to the initial lesion, whatever the initial grade of the primary tumor. ASPM expression also increased over serial passages in gliomaspheres in vitro and in mouse xenografts in vivo. Lentivirus-mediated shRNA silencing of ASPM resulted in dramatic proliferation arrest and cell death in two different gliomasphere models. CONCLUSION: These data suggest that ASPM is involved in the malignant progression of gliomas, possibly through expansion of a cancer stem cell compartment, and is an attractive therapeutic target in glioblastoma multiforme.
Type de document :
Article dans une revue
Cancer Cell International, BioMed Central, 2010, 10 (1), pp.1. 〈10.1186/1475-2867-10-1〉
Liste complète des métadonnées

Littérature citée [12 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-00668477
Contributeur : Ed. Bmc <>
Soumis le : jeudi 9 février 2012 - 17:23:21
Dernière modification le : vendredi 31 août 2018 - 09:13:20
Document(s) archivé(s) le : jeudi 10 mai 2012 - 02:59:32

Fichiers

1475-2867-10-1.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

UNIV-AMU | UPMC | ICM

Citation

Sandra-Nadia Bikeye, Carole Colin, Yannick Marie, Raphaël Vampouille, Philippe Ravassard, et al.. ASPM-associated stem cell proliferation is involved in malignant progression of gliomas and constitutes an attractive therapeutic target.. Cancer Cell International, BioMed Central, 2010, 10 (1), pp.1. 〈10.1186/1475-2867-10-1〉. 〈inserm-00668477〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

441

Téléchargements de fichiers

308

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD