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Journal articles
Carbon black and titanium dioxide nanoparticles elicit distinct apoptotic pathways in bronchial epithelial cells.
Abstract : BACKGROUND: Increasing environmental and occupational exposures to nanoparticles (NPs) warrant deeper insight into the toxicological mechanisms induced by these materials. The present study was designed to characterize the cell death induced by carbon black (CB) and titanium dioxide (TiO2) NPs in bronchial epithelial cells (16HBE14o- cell line and primary cells) and to investigate the implicated molecular pathways. RESULTS: Detailed time course studies revealed that both CB (13 nm) and TiO2(15 nm) NP exposed cells exhibit typical morphological (decreased cell size, membrane blebbing, peripheral chromatin condensation, apoptotic body formation) and biochemical (caspase activation and DNA fragmentation) features of apoptotic cell death. A decrease in mitochondrial membrane potential, activation of Bax and release of cytochrome c from mitochondria were only observed in case of CB NPs whereas lipid peroxidation, lysosomal membrane destabilization and cathepsin B release were observed during the apoptotic process induced by TiO2 NPs. Furthermore, ROS production was observed after exposure to CB and TiO2 but hydrogen peroxide (H2O2) production was only involved in apoptosis induction by CB NPs. CONCLUSIONS: Both CB and TiO2 NPs induce apoptotic cell death in bronchial epithelial cells. CB NPs induce apoptosis by a ROS dependent mitochondrial pathway whereas TiO2 NPs induce cell death through lysosomal membrane destabilization and lipid peroxidation. Although the final outcome is similar (apoptosis), the molecular pathways activated by NPs differ depending upon the chemical nature of the NPs.
Cited literature [66 references]
https://www.hal.inserm.fr/inserm-00668461
Contributor : Ed. Bmc Connect in order to contact the contributor
Submitted on : Thursday, February 9, 2012 - 5:18:48 PM
Last modification on : Thursday, December 16, 2021 - 5:18:01 PM
Long-term archiving on: : Thursday, May 10, 2012 - 2:59:12 AM
Contributor : Ed. Bmc Connect in order to contact the contributor
Submitted on : Thursday, February 9, 2012 - 5:18:48 PM
Last modification on : Thursday, December 16, 2021 - 5:18:01 PM
Long-term archiving on: : Thursday, May 10, 2012 - 2:59:12 AM