The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac. - Archive ouverte HAL Access content directly
Journal Articles BMC Biochemistry Year : 2010

The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac.

(1) , (1, 2) , (3) , (4) , (1, 5) , (1, 5)
1
2
3
4
5

Abstract

BACKGROUND: RhoGDI proteins are important regulators of the small GTPase Rac, because they shuttle Rac from the cytoplasm to membranes and also protect Rac from activation, deactivation and degradation. How the binding and release of Rac from RhoGDI is regulated is not precisely understood. RESULTS: We report that the non-receptor tyrosine kinase Fer is able to phosphorylate RhoGDIα and form a direct protein complex with it. This interaction is mediated by the C-terminal end of RhoGDIα. Activation of Fer by reactive oxygen species caused increased phosphorylation of RhoGDIα and pervanadate treatment further augmented this. Tyrosine phosphorylation of RhoGDIα by Fer prevented subsequent binding of Rac to RhoGDIα, but once a RhoGDIα-Rac complex was formed, the Fer kinase was not able to cause Rac release through tyrosine phosphorylation of preformed RhoGDIα-Rac complexes. CONCLUSIONS: These results identify tyrosine phosphorylation of RhoGDIα by Fer as a mechanism to regulate binding of RhoGDIα to Rac.
Fichier principal
Vignette du fichier
1471-2091-11-48.pdf (603.15 Ko) Télécharger le fichier
Vignette du fichier
1471-2091-11-48.xml (36.47 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Other
Loading...

Dates and versions

inserm-00668427 , version 1 (09-02-2012)

Identifiers

Cite

Fei Fei, Soo-Mi Kweon, Leena Haataja, Paulo de Sepulveda, John Groffen, et al.. The Fer tyrosine kinase regulates interactions of Rho GDP-Dissociation Inhibitor α with the small GTPase Rac.. BMC Biochemistry, 2010, 11 (1), pp.48. ⟨10.1186/1471-2091-11-48⟩. ⟨inserm-00668427⟩

Collections

INSERM CNRS
99 View
304 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More