Enterocytic differentiation is modulated by lipid rafts-dependent assembly of adherens junctions.: lipid raft and enterocytic differentiation

Nicolas Chartier 1, 2, 3 Michèle Lainé 4 Benjamin Ducarouge 5 Christiane Oddou 1, 6 Bruno Bonaz 7 Corinne Albiges-Rizo 8 Muriel Jacquier-Sarlin 4, *
* Auteur correspondant
4 INSERM U836, équipe 8, Stress et interactions neurodigestives
DySAD - Dynamique des systèmes d'adhérence et différenciation, GIN - Grenoble Institut des Neurosciences
5 INSERM U836, équipe 8, Stress et interactions neurodigestives
UJF - Université Joseph Fourier - Grenoble 1, DySAD - Dynamique des systèmes d'adhérence et différenciation
7 INSERM U836, équipe 8, Stress et interactions neurodigestives
GIN - Grenoble Institut des Neurosciences
8 INSERM U823, équipe 1 (DySAD)
UJF - Université Joseph Fourier - Grenoble 1, DySAD - Dynamique des systèmes d'adhérence et différenciation, Equipe de Recherche Labellisée 3148
Abstract : Integrity of the epithelial barrier is determined by apical junctional complexes which also participate in the signalling pathways inducing intestinal cell differentiation. Lipid rafts (LR) have been proposed to play a role in the organization and the function of these intercellular complexes. This study investigated potential mechanisms by which LR could participate in the establishment of adherens junctions (AJ) and the initiation of enterocytic differentiation. We showed that the differentiation of epithelial cells in rat colons correlates with the emergence of LR. Using HT-29 cells we demonstrated that during the differentiation process, LR are required for the recruitment and the association of p120ctn to E-cadherin. Silencing of flotillin-1, a LR component, alters the recruitment of AJ proteins in LR and delays the expression of differentiation markers. Furthermore, the ability of p120ctn/E-cadherin complexes to support cell differentiation is altered in HT-29 Rac1N17 cells. These results show a contributory role of LR in the enterocytic differentiation process, which serve as signalling platforms for Rac1-mediated organization of AJ. A better understanding of the mechanism involved in the establishment of junctional complex and their role in enterocytic differentiation provides new insights into the regulation of intestinal homeostasis.
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Experimental Cell Research, Elsevier, 2011, 317 (10), pp.1422-36. 〈10.1016/j.yexcr.2011.03.009〉
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Nicolas Chartier, Michèle Lainé, Benjamin Ducarouge, Christiane Oddou, Bruno Bonaz, et al.. Enterocytic differentiation is modulated by lipid rafts-dependent assembly of adherens junctions.: lipid raft and enterocytic differentiation. Experimental Cell Research, Elsevier, 2011, 317 (10), pp.1422-36. 〈10.1016/j.yexcr.2011.03.009〉. 〈inserm-00668336〉

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