Four-color flow cytometry bypasses limitations of IG/TCR polymerase chain reaction for minimal residual disease detection in certain subsets of children with acute lymphoblastic leukemia.

Nelly Robillard 1 Hélène Cavé 2 Françoise Méchinaud 3 Christine Guidal 2 Francine Garnache-Ottou 4 Pierre-Simon Rohrlich 5 Hervé Avet-Loiseau 1 Richard Garand 1, *
* Auteur correspondant
1 Service d'hématologie clinique
2 Laboratoire de Biochimie Génétique
3 Service d'Oncologie Hématologie Pédiatrique
4 HOTE GREFFON - Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098)
5 Service d'Hématologie Pédiatrique
Abstract : BACKGROUND AND OBJECTIVES: Competitive immunoglobulin/T-cell receptor polymerase-chain reaction (PCR) analysis with fluorescent detection is a rapid, cheap and reproducible method for quantifying minimal residual disease (MRD), which is well adapted to the recognition of high-risk childhood acute lymphoblastic leukemia (ALL). We aimed at defining whether flow cytometry (FC) techniques can bypass limitations of PCR for MRD determination. DESIGN AND METHODS: We analyzed 140 remission samples from 91 patients using both competitive PCR amplification of antigen-receptor genes and four-color FC identification of leukemia immunophenotype. These methods were chosen with the aim of detecting at least 0.1% blasts. RESULTS: MRD was measured using both PCR and FC methods in 123 samples and the two methods provided concordant results in 119 of them (97%). Moreover, three out of the four discordant results appeared minor since MRD was detectable by both methods, but at different levels. In 12 of 13 samples from nine patients, mainly infants with early CD10- and/or t(4;11) B-cell ALL and children with immature T-cell ALL, MRD could be determined using FC whereas PCR failed. Conversely, FC methods were unfeasible due to inappropriate leukemia immunophenotype in three additional children (including two with T-cell ALL) for whom PCR successfully provided MRD results. INTERPRETATION AND CONCLUSIONS: The MRD results provided by FC techniques were highly concordant with those of competitive PCR. Moreover, the applicability of FC appeared higher in certain ALL subsets, although the appropriateness of this technique in terms of outcome prediction remains to be demonstrated.
Keywords : minimal residual disease acute lymphoblastic leukemia flow cytometry PCR minimal residual disease.
Type de document :
Article dans une revue
Haematologica, Ferrata Storti Foundation, 2005, 90 (11), pp.1516-23
Liste complète des métadonnées
http://www.hal.inserm.fr/inserm-00668267
Contributeur : Philippe Saas <>
Soumis le : jeudi 9 février 2012 - 14:36:47
Dernière modification le : lundi 9 juillet 2018 - 13:12:02
Document(s) archivé(s) le : lundi 14 mai 2012 - 13:19:56

Fichier

1516.full.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

  • HAL Id : inserm-00668267, version 1
  • PUBMED : 16266899

Collections

UNIV-FCOMTE | UMR645

Citation

Nelly Robillard, Hélène Cavé, Françoise Méchinaud, Christine Guidal, Francine Garnache-Ottou, et al.. Four-color flow cytometry bypasses limitations of IG/TCR polymerase chain reaction for minimal residual disease detection in certain subsets of children with acute lymphoblastic leukemia.. Haematologica, Ferrata Storti Foundation, 2005, 90 (11), pp.1516-23. 〈inserm-00668267〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

259

Téléchargements de fichiers

215

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD