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Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients.

Abstract : OBJECTIVES: Interleukin (IL) 34 is a new cytokine implicated in macrophage differentiation and osteoclastogenesis. This study assessed IL-34 expression in the tissue of patients with rheumatoid arthritis (RA). METHODS: Immunohistochemistry was performed in synovial biopsies from patients with RA (n=20), osteoarthritis (n=3) or other inflammatory arthritis (n=4). IL-34 was detected in the synovial fluid by ELISA and its messenger RNA expression was studied by quantitative PCR in rheumatoid synovial fibroblasts after stimulation by tumour necrosis factor α (TNFα) and IL-1β. Wild-type, jnk1(-/-)-jnk2(-/-) and nemo(-/-) murine fibroblasts and pharmacological inhibition were used to determine the involvement of nuclear factor kappa B (NF-κB) and JNK in that effect. RESULTS: IL-34 was expressed in 24/27 biopsies, with three samples from RA patients being negative. A significant association was found between IL-34 expression and synovitis severity. Levels of IL-34 and the total leucocyte count in synovial fluid were correlated. TNFα and IL-1β stimulated IL-34 expression by synovial fibroblasts in a dose/time-dependent manner through the NF-κB and JNK pathway. CONCLUSION: This work for the first time identifies IL-34 expression in the synovial tissue of patients with arthritis. This cytokine, as a downstream effector of TNFα and IL-1β, may contribute to inflammation and bone erosions in RA.
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https://www.hal.inserm.fr/inserm-00667486
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Submitted on : Tuesday, February 7, 2012 - 5:11:23 PM
Last modification on : Thursday, February 7, 2019 - 5:10:44 PM
Long-term archiving on: : Tuesday, May 8, 2012 - 2:35:14 AM

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M. Chemel, Benoît Le Goff, Régis Brion, C. Cozic, Martine Berreur, et al.. Interleukin 34 expression is associated with synovitis severity in rheumatoid arthritis patients.. Annals of the Rheumatic Diseases, BMJ Publishing Group, 2012, 71 (1), pp.150-4. ⟨10.1136/annrheumdis-2011-200096⟩. ⟨inserm-00667486⟩

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