Characterization of intratumoral follicular helper T cells in follicular lymphoma: role in the survival of malignant B cells.: Intratumoral TFH in follicular lymphoma

Abstract : Accumulating evidences indicate that the cellular and molecular microenvironment of follicular lymphoma (FL) has a key role in both lymphomagenesis and patient outcome. Malignant FL B cells are found admixed to specific stromal and immune cell subsets, in particular CD4(pos) T cells displaying phenotypic features of follicular helper T cells (T(FH)). The goal of our study was to functionally characterize intratumoral CD4(pos) T cells. We showed that CXCR5(hi)ICOS(hi)CD4(pos) T cells sorted from FL biopsies comprise at least two separate cell populations with distinct genetic and functional features: (i) CD25(pos) follicular regulatory T cells (T(FR)), and (ii) CD25(neg) T(FH) displaying a FL-B cell supportive activity without regulatory functions. Furthermore, despite their strong similarities with tonsil-derived T(FH), purified FL-derived T(FH) displayed a specific gene expression profile including an overexpression of several genes potentially involved directly or indirectly in lymphomagenesis, in particular TNF, LTA, IL4 or CD40LG. Interestingly, we further demonstrated that these two last signals efficiently rescued malignant B cells from spontaneous and rituximab-induced apoptosis. Altogether, our study demonstrates that tumor-infiltrating CD4(pos) T cells are more heterogeneous than previously presumed, and underlines for the first time the crucial role of T(FH) in the complex set of cellular interactions within FL microenvironment.
Type de document :
Article dans une revue
Leukemia, Nature Publishing Group: Open Access Hybrid Model Option B, 2012, 26 (5), pp.1053-63. 〈10.1038/leu.2011.301〉
Liste complète des métadonnées

Littérature citée [6 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00666043
Contributeur : Patricia Amé-Thomas <>
Soumis le : mardi 24 avril 2012 - 15:28:51
Dernière modification le : jeudi 5 juillet 2018 - 16:34:02

Fichiers

Leukemia_2011_HAL.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Citation

Patricia Amé-Thomas, Jérôme Le Priol, Hans Yssel, Gersende Caron, Céline Pangault, et al.. Characterization of intratumoral follicular helper T cells in follicular lymphoma: role in the survival of malignant B cells.: Intratumoral TFH in follicular lymphoma. Leukemia, Nature Publishing Group: Open Access Hybrid Model Option B, 2012, 26 (5), pp.1053-63. 〈10.1038/leu.2011.301〉. 〈inserm-00666043〉

Partager

Métriques

Consultations de la notice

326

Téléchargements de fichiers

628