Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization. - Archive ouverte HAL Access content directly
Journal Articles AIDS Research and Therapy Year : 2010

Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization.

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Mohammad-Ali Jenabian
  • Function : Author
  • PersonId : 919077
Héla Saïdi
  • Function : Author
  • PersonId : 919078
Charlotte Charpentier
  • Function : Author
  • PersonId : 919079
Thomas Bell
  • Function : Author
  • PersonId : 919083
Guido Vanham
  • Function : Author
  • PersonId : 909067
Laurent Bélec
  • Function : Correspondent author
  • PersonId : 919084

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Abstract

BACKGROUND: HIV-1 in genital secretions may be opsonized by several molecules including complement components. Opsonized HIV-1 by complement enhances the infection of various mucosal target cells, such as dendritic cells (DC) and epithelial cells. RESULTS: We herein evaluated the effect of HIV-1 complement opsonization on microbicide candidates' activity, by using three in vitro mucosal models: CCR5-tropic HIV-1JR-CSF transcytosis through epithelial cells, HIV-1JR-CSF attachment on immature monocyte-derived dendritic cells (iMDDC), and infectivity of iMDDC by CCR5-tropic HIV-1BaL and CXCR4-tropic HIV-1NDK. A panel of 10 microbicide candidates [T20, CADA, lectines HHA & GNA, PVAS, human lactoferrin, and monoclonal antibodies IgG1B12, 12G5, 2G12 and 2F5], were investigated using cell-free unopsonized or opsonized HIV-1 by complements. Only HHA and PVAS were able to inhibit HIV trancytosis. Upon opsonization, transcytosis was affected only by HHA, HIV-1 adsorption on iMDDC by four molecules (lactoferrin, IgG1B12, IgG2G5, IgG2G12), and replication in iMDDC of HIV-1BaL by five molecules (lactoferrin, CADA, T20, IgG1B12, IgG2F5) and of HIV-1NDK by two molecules (lactoferrin, IgG12G5). CONCLUSION: These observations demonstrate that HIV-1 opsonization by complements may modulate in vitro the efficiency of candidate microbicides to inhibit HIV-1 infection of mucosal target cells, as well as its crossing through mucosa.
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inserm-00663893 , version 1 (27-01-2012)

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Mohammad-Ali Jenabian, Héla Saïdi, Charlotte Charpentier, Hicham Bouhlal, Dominique Schols, et al.. Differential activity of candidate microbicides against early steps of HIV-1 infection upon complement virus opsonization.. AIDS Research and Therapy, 2010, 7 (1), pp.16. ⟨10.1186/1742-6405-7-16⟩. ⟨inserm-00663893⟩
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