Papillomavirus pseudovirions packaged with the L2 gene induce cross-neutralizing antibodies.

Nicolas Combelas 1 Emilie Saussereau 1 Maxime Fleury 1 Tatiana Ribeiro 1 Julien Gaitan 1 Diego Duarte-Forero 1, 2 Pierre Coursaget 1, * Antoine Touzé 1
* Auteur correspondant
1 Pathologies Respiratoires : Protéolyse et Aérosolthérapie
2 Instituto Nacional de Cancerologia
Abstract : BACKGROUND: Current vaccines against HPVs are constituted of L1 protein self-assembled into virus-like particles (VLPs) and they have been shown to protect against natural HPV16 and HPV18 infections and associated lesions. In addition, limited cross-protection has been observed against closely related types. Immunization with L2 protein in animal models has been shown to provide cross-protection against distant papillomavirus types, suggesting that the L2 protein contains cross-neutralizing epitopes. However, vaccination with L2 protein or L2 peptides does not induce high titers of anti-L2 antibodies. In order to develop a vaccine with the potential to protect against other high-risk HPV types, we have produced HPV58 pseudovirions encoding the HPV31 L2 protein and compared their capacity to induce cross-neutralizing antibodies with that of HPV L1 and HPV L1/L2 VLPs. METHODS: The titers of neutralizing antibodies against HPV16, HPV18, HPV31 and HPV58 induced in Balb/c mice were compared after immunization with L2-containing vaccines. RESULTS: Low titers of cross-neutralizing antibodies were detected in mice when immunized with L1/L2 VLPs, and the highest levels of cross-neutralizing antibodies were observed in mice immunized with HPV 58 L1/L2 pseudovirions encoding the HPV 31 L2 protein. CONCLUSIONS: The results obtained indicate that high levels of cross-neutralizing antibodies are only observed after immunization with pseudovirions encoding the L2 protein. HPV pseudovirions thus represent a possible new strategy for the generation of a broad-spectrum vaccine to protect against high-risk HPVs and associated neoplasia.
Type de document :
Article dans une revue
Journal of Translational Medicine, BioMed Central, 2010, 8 (1), pp.28. 〈10.1186/1479-5876-8-28〉
Liste complète des métadonnées

Littérature citée [43 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-00663763
Contributeur : Ed. Bmc <>
Soumis le : vendredi 27 janvier 2012 - 14:23:55
Dernière modification le : mercredi 21 mars 2018 - 10:54:03
Document(s) archivé(s) le : lundi 19 novembre 2012 - 15:10:23

Fichiers

1479-5876-8-28.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Nicolas Combelas, Emilie Saussereau, Maxime Fleury, Tatiana Ribeiro, Julien Gaitan, et al.. Papillomavirus pseudovirions packaged with the L2 gene induce cross-neutralizing antibodies.. Journal of Translational Medicine, BioMed Central, 2010, 8 (1), pp.28. 〈10.1186/1479-5876-8-28〉. 〈inserm-00663763〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

265

Téléchargements de fichiers

356

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD