In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biomedical Science Year : 2010

In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles.

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Meng-Ing Chou
  • Function : Author
  • PersonId : 918915
Yu-Fan Hsieh
  • Function : Author
  • PersonId : 918916
Meilin Wang
  • Function : Author
  • PersonId : 918917
Deching Chang
  • Function : Author
  • PersonId : 918919
Moncef Zouali
  • Function : Author
  • PersonId : 918920

Abstract

BACKGROUND: RNA interference (RNAi) is a powerful tool to silence gene expression post-transcriptionally. Delivering sequences of RNAi in vivo remains a problem. The aim of this study was to use JC virus (JCV) virus-like particles (VLPs) as a vector for delivering RNAi in silencing the cytokine gene of IL-10. METHODS: JCV VLPs were generated by recombinant JCV VP1 protein in yeast expression system. DNA fragment containing IL-10 shRNA was packaged into VLPs by osmotic shock. RESULTS: In RAW 264.7 cells, IL-10 shRNA was found to reduce IL-10 expression by 85 to 89%, as compared with VLPs alone. IL-10 shRNA did not cross-react with TNF-alpha mRNA or influence the expression of TNF-alpha. In BALB/c mice IL-10 shRNA could reduce 95% of IL-10 secretion. Surprisingly, it also down regulated TNF-alpha expression. CONCLUSIONS: We show for the first time that JCV VLPs empty capsids are competent vectors to deliver RNAi and are nontoxic to cells, suggesting that JCV VLPs is an efficient agent to deliver RNAi in both murine macrophage cells and BALB/c mice. This system provides an efficient means for delivering the RNAi for gene therapy purposes.
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Dates and versions

inserm-00663735 , version 1 (27-01-2012)

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Meng-Ing Chou, Yu-Fan Hsieh, Meilin Wang, Jinghua Tsai Chang, Deching Chang, et al.. In vitro and in vivo targeted delivery of IL-10 interfering RNA by JC virus-like particles.. Journal of Biomedical Science, 2010, 17 (1), pp.51. ⟨10.1186/1423-0127-17-51⟩. ⟨inserm-00663735⟩
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