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Analysis of cellular signaling events by flow cytometry

Abstract : All the cells are engaged on a cell communication by sending and receiving signals. Cells having the correct receptors on their surfaces will encode the plasma membrane signal recognition to intracellular signals corresponding to cellular signaling events. This process corresponds to a huge intracellular network of protein-protein or lipid-protein interactions. To avoid to be lost in this kind of maze, some general signaling pathways are most of the time present in many cell systems such as MAP kinases or PI-3 kinases pathways. These signaling pathways induce some protein phosphorylation events that can be identified by specific antibodies directed against these phosphosites (anti-phospho antibodies). Thus, these crossroads can be determined by using antibodies. A widely used method to identify these signaling events is the Western blotting or protein immunoblotting from cell extracts. However, several research groups are now developing new analytical techniques based also on the use of anti-phospho antibodies, but allowing them to detect signaling events at the single cell level. These experimental approaches are using the principles of the flow cytometry (FCM). Phosphoflow technology will provide rapid, quantitative and also multi-parameter analyses on single cells.
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Contributor : Jacques Nunès Connect in order to contact the contributor
Submitted on : Friday, June 15, 2012 - 11:05:06 AM
Last modification on : Friday, October 22, 2021 - 3:29:07 AM
Long-term archiving on: : Sunday, September 16, 2012 - 2:31:20 AM


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Jacques A. Nunès, Guylène Firaguay, Emilie Coppin. Analysis of cellular signaling events by flow cytometry. Dr. Ingrid Schmid. Flow Cytometry - Recent Perspectives, InTech, pp.1-12, 2012, ⟨10.5772/38012⟩. ⟨inserm-00661295v2⟩



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