Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.

Aurélie Lardenois 1 Frédéric Chalmel 1 Philippe Demougin 2 Noora Kotaja 3 Paolo Sassone-Corsi 4 Michael Primig 1, *
* Auteur correspondant
1 GERHM - Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes
2 Biozentrum
3 University of Turku
4 University of California
Abstract : BACKGROUND: The LIM domain protein Fhl5 was previously found to interact with CREM, a DNA binding transcriptional regulator necessary for spermiogenesis in mammals. Co-transfection experiments using heterologous promoter constructs indicated a role for Fhl5 in transcriptional up-regulation of CREM-dependent testicular genes. Male mice lacking Fhl5 were reported to be fertile but displayed partially abnormal sperm maturation and morphology. METHODS: To identify Fhl5 testicular target genes we carried out two whole-genome expression profiling experiments using high-density oligonucleotide microarrays and total testis samples from Fhl5 wild-type versus homozygous mutant mice first in different and then in isogenic strain backgrounds. RESULTS: Weak signal differences were detected in non-isogenic samples but no statistically significant expression changes were observed when isogenic Fhl5 mutant and wild-type samples were compared. CONCLUSION: The outcome of these experiments suggests that testicular expression profiling is extremely sensitive to the genetic background and that Fhl5 is not essential for testicular gene expression to a level detected by microarray-based measurements. This might be due to redundant function of the related and similarly expressed protein Fhl4.
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Reproductive Biology and Endocrinology, BioMed Central, 2009, 7 (1), pp.133. 〈10.1186/1477-7827-7-133〉
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Aurélie Lardenois, Frédéric Chalmel, Philippe Demougin, Noora Kotaja, Paolo Sassone-Corsi, et al.. Fhl5/Act, a CREM-binding transcriptional activator required for normal sperm maturation and morphology, is not essential for testicular gene expression.. Reproductive Biology and Endocrinology, BioMed Central, 2009, 7 (1), pp.133. 〈10.1186/1477-7827-7-133〉. 〈inserm-00658993〉

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