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Vessel size index measurements in a rat model of glioma: comparison of the dynamic (Gd) and steady-state (iron-oxide) susceptibility contrast MRI approaches.

Nicolas Pannetier 1 Benjamin Lemasson 2 Thomas Christen 1 Mohamed Tachrount 1 Irène Troprès 3 Régine Farion 1 Christoph Segebarth 1 Chantal Rémy 1 Emmanuel Barbier 1, *
* Corresponding author
2 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
GIN - Grenoble Institut des Neurosciences, Oncodesign Inc.
3 INSERM U836, équipe 5, Neuroimagerie fonctionnelle et perfusion cérébrale
RBCH - RMN biomédicale : de la cellule à l'homme, GIN - Grenoble Institut des Neurosciences
Abstract : Vessel size index (VSI), a parameter related to the distribution of vessel diameters, may be estimated using two MRI approaches: (i) dynamic susceptibility contrast (DSC) MRI following the injection of a bolus of Gd-chelate. This technique is routinely applied in the clinic to assess intracranial tissue perfusion in patients; (ii) steady-state susceptibility contrast with USPIO contrast agents, which is considered here as the standard method. Such agents are not available for human yet and the steady-state approach is currently limited to animal studies. The aim is to compare VSI estimates obtained with these two approaches on rats bearing C6 glioma (n = 7). In a first session, VSI was estimated from two consecutive injections of Gd-Chelate (Gd(1) and Gd(2)). In a second session (4 hours later), VSI was estimated using USPIO. Our findings indicate that both approaches yield comparable VSI estimates both in contralateral (VSI{USPIO} = 7.5 ± 2.0 µm, VSI{Gd(1)} = 6.5 ± 0.7 µm) and in brain tumour tissues (VSI{USPIO} = 19.4 ± 7.1 µm, VSI{Gd(1)} = 16.6 ± 4.5 µm). We also observed that, in the presence of BBB leakage (as it occurs typically in brain tumours), applying a preload of Gd-chelate improves the VSI estimate with the DSC approach both in contralateral (VSI{Gd(2)} = 7.1 ± 0.4 µm) and in brain tumour tissues (VSI{Gd(2)} = 18.5 ± 4.3 µm) but is not mandatory. VSI estimates do not appear to be sensitive to T(1) changes related to Gd extravasation. These results suggest that robust VSI estimates may be obtained in patients at 3 T or higher magnetic fields with the DSC approach.
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Submitted on : Tuesday, January 10, 2012 - 4:13:06 PM
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Nicolas Pannetier, Benjamin Lemasson, Thomas Christen, Mohamed Tachrount, Irène Troprès, et al.. Vessel size index measurements in a rat model of glioma: comparison of the dynamic (Gd) and steady-state (iron-oxide) susceptibility contrast MRI approaches.. NMR in Biomedicine, Wiley, 2012, 25 (2), pp.218-26. ⟨10.1002/nbm.1734⟩. ⟨inserm-00658563⟩

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