Study of possible interactions of tubulin, microtubular network, and STOP protein with mitochondria in muscle cells.

Karen Guerrero 1 Claire Monge 1 Anna Brückner 1 Ulo Puurand 2 Lumme Kadaja 2 Tuuli Käämbre 3 Enn Seppet 2 Valdur Saks 1, 3, *
* Auteur correspondant
1 Bioénergétique fondamentale et appliquée
2 Department of Pathophysiology
3 Laboratory of Bioenergetics
Abstract : We studied possible connections of tubulin, microtubular system, and microtubular network stabilizing STOP protein with mitochondria in rat and mouse cardiac and skeletal muscles by confocal microscopy and oxygraphy. Intracellular localization and content of tubulin was found to be muscle type-specific, with high amounts in oxidative muscles, and much lower in glycolytic skeletal muscle. STOP protein localization and content in muscle cells was also muscle type-specific. In isolated heart mitochondria, addition of 1 microM tubulin heterodimer increased apparent K(m) for ADP significantly. Dissociation of microtubular system into free tubulin by colchicine treatment only slightly decreased initially high apparent K(m) for ADP in permeabilized cells, and diffusely distributed free tubulin stayed inside the cells, obviously connected to the intracellular structures. To identify the genes that are specific for oxidative muscle, we developed and applied a method of kindred DNA. The results of sequencing and bioinformatic analysis of isolated cDNA pool common for heart and m. soleus showed that in adult mice the beta-tubulin gene is expressed predominantly in oxidative muscle cells. It is concluded that whereas dimeric tubulin may play a significant role in regulation of mitochondrial outer membrane permeability in the cells in vivo, its organization into microtubular network has a minor significance on that process.
Keywords : Cytoskeleton Mitochondria Oxidative phosphorylation Tubulin Microtubular Network STOP protein Heart Skeletal muscles
Type de document :
Article dans une revue
Molecular and Cellular Biochemistry, Springer Verlag, 2010, 337 (1-2), pp.239-49. 〈10.1007/s11010-009-0304-1〉
Liste complète des métadonnées
http://www.hal.inserm.fr/inserm-00628900
Contributeur : Sarah Hamant <>
Soumis le : mardi 4 octobre 2011 - 14:46:43
Dernière modification le : jeudi 6 octobre 2011 - 14:46:15

Lien texte intégral

istex

Identifiants

Collections

UGA

Citation

Karen Guerrero, Claire Monge, Anna Brückner, Ulo Puurand, Lumme Kadaja, et al.. Study of possible interactions of tubulin, microtubular network, and STOP protein with mitochondria in muscle cells.. Molecular and Cellular Biochemistry, Springer Verlag, 2010, 337 (1-2), pp.239-49. 〈10.1007/s11010-009-0304-1〉. 〈inserm-00628900〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

120

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD