Identification of small RNAs in Francisella tularensis.

Abstract : BACKGROUND: Regulation of bacterial gene expression by small RNAs (sRNAs) have proved to be important for many biological processes. Francisella tularensis is a highly pathogenic Gram-negative bacterium that causes the disease tularaemia in humans and animals. Relatively little is known about the regulatory networks existing in this organism that allows it to survive in a wide array of environments and no sRNA regulators have been identified so far. RESULTS: We have used a combination of experimental assays and in silico prediction to identify sRNAs in F. tularensis strain LVS. Using a cDNA cloning and sequencing approach we have shown that F. tularensis expresses homologues of several sRNAs that are well-conserved among diverse bacteria. We have also discovered two abundant putative sRNAs that share no sequence similarity or conserved genomic context with any previously annotated regulatory transcripts. Deletion of either of these two loci led to significant changes in the expression of several mRNAs that likely include the cognate target(s) of these sRNAs. Deletion of these sRNAs did not, however, significantly alter F. tularensis growth under various stress conditions in vitro, its replication in murine cells, or its ability to induce disease in a mouse model of F. tularensis infection. We also conducted a genome-wide in silico search for intergenic loci that suggests F. tularensis encodes several other sRNAs in addition to the sRNAs found in our experimental screen. CONCLUSION: Our findings suggest that F. tularensis encodes a significant number of non-coding regulatory RNAs, including members of well conserved families of structural and housekeeping RNAs and other poorly conserved transcripts that may have evolved more recently to help F. tularensis deal with the unique and diverse set of environments with which it must contend.
Type de document :
Article dans une revue
BMC Genomics, BioMed Central, 2010, 11 (1), pp.625. 〈10.1186/1471-2164-11-625〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00622785
Contributeur : Ed. Bmc <>
Soumis le : lundi 12 septembre 2011 - 16:33:56
Dernière modification le : vendredi 14 septembre 2018 - 14:30:03
Document(s) archivé(s) le : mardi 13 novembre 2012 - 10:25:50

Identifiants

Collections

Citation

Guillaume Postic, Eric Frapy, Marion Dupuis, Iharilalao Dubail, Jonathan Livny, et al.. Identification of small RNAs in Francisella tularensis.. BMC Genomics, BioMed Central, 2010, 11 (1), pp.625. 〈10.1186/1471-2164-11-625〉. 〈inserm-00622785〉

Partager

Métriques

Consultations de la notice

175

Téléchargements de fichiers

242