Skip to Main content Skip to Navigation
Journal articles

Differential role for CD277 as a co-regulator of the immune signal in T and NK cells.

Abstract : The human butyrophilin (BTN) 3 or CD277 molecules belong to the B7 family members and are expressed in various immune cells such as T and NK cells. Here, we show that CD277 triggering considerably enhances TCR-induced cytokine production and cell proliferation, even when another co-stimulatory molecule, CD28, is engaged. These CD277-induced additive functional effects are in accordance with the detection of early T-cell activation events such as TCR-induced cell signaling being increased upon CD277 engagement. However, we found that CD277 triggering is not involved in CD16- or NKp46-induced NK cell activation. BTN3/CD277 comprises three structurally related members, BTN3A1, BTN3A2 and BTN3A3. CD277 antibodies recognize all isoforms and we describe a differential expression of BTN3 isoforms between T and NK cells that could explain differential CD277 functions between T and NK cells. Our results show that, while T cells express all BTN3/CD277 transcripts, NK cells express mostly BTN3A2, which lacks the B30.2 intracellular domain. Furthermore, NKp30-induced cytokine production is decreased by the specific engagement of BTN3A2, but not by BTN3A1 triggering. Thus, we provide new insights into the CD277 co-stimulatory pathway that may differentially participate in the regulation of various cell-mediated immune responses.
Document type :
Journal articles
Complete list of metadatas

Cited literature [26 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00620920
Contributor : Jacques Nunès <>
Submitted on : Monday, September 19, 2011 - 9:00:15 AM
Last modification on : Thursday, December 20, 2018 - 11:19:07 AM
Long-term archiving on: : Tuesday, December 20, 2011 - 2:20:22 AM

File

CD277_manuscript.pdf.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Nassima Messal, Emilie Mamessier, Aude Sylvain, Javier Celis Gutierrez, Marie-Laure Thibult, et al.. Differential role for CD277 as a co-regulator of the immune signal in T and NK cells.. European Journal of Immunology, Wiley-VCH Verlag, 2011, 41 (12), pp.3443-54. ⟨10.1002/eji.201141404⟩. ⟨inserm-00620920⟩

Share

Metrics

Record views

1115

Files downloads

1542