Skeletal muscle lipase content and activity in obesity and type 2 diabetes.

Johan Jocken 1, * Cedric Moro 2 Gijs Goossens 1 Dominique Hansen 3 Aline Mairal 2 Matthijs Hesselink 4 Dominique Langin 2 Luc Van Loon 4 Ellen Blaak 1
* Auteur correspondant
1 Department of Human Biology
2 I2MR - Institut de médecine moléculaire de Rangueil
3 Jessa Hospital and Rehabilitation and Healthcare Research Center
4 Department of Human Movement Sciences
Abstract : CONTEXT: The obese insulin-resistant state is characterized by elevated lipid storage in skeletal muscle tissue. OBJECTIVE: We tested whether differences in muscle triacylglycerol (TAG) and diacylglycerol (DAG) lipase content and activity are associated with incomplete in vivo lipolysis and lipid accumulation. DESIGN AND PATIENTS: Two case-control studies were conducted on skeletal muscle biopsies from lean (n=13) and obese (n=10) men (study 1) and from 11 nonobese type 2 diabetic (T2D), obese T2D, and healthy normoglycemic men (study 2). MAIN OUTCOME MEASURES: Skeletal muscle lipase protein content and activity and muscle lipid content (TAG and DAG) were determined. RESULTS: Skeletal muscle hormone-sensitive lipase protein content was lower (0.39±0.07 vs. 1.00±0.19 arbitrary units; P=0.004) and adipose triglyceride lipase protein content was higher in obese men compared with lean controls (2.17±0.40 vs. 0.42±0.23 arbitrary units; P=0.008). This apparent difference in lipase content was accompanied by a 60% lower ratio of DAG to TAG hydrolase activity in the obese men (11.4±2.3 vs. 26.5±7.3 nmol/h*mg; P=0.045), implying incomplete lipolysis. Lower hormone-sensitive lipase and higher adipose triglyceride lipase content was confined to obesity per se, because it was observed solely in obese T2D men but not in healthy normoglycemic controls and nonobese T2D men. Muscle total DAG content was not higher in obese men but was even lower (6.2±0.7 vs. 9.4±0.9 μmol/mg dry weight; P=0.017). TAG content did not differ between groups (84.7±18.9 vs. 70.4±12.4 μmol/mg dry weight; P=0.543). CONCLUSIONS: Our data do not support an important role of total muscle DAG content in the development of insulin resistance in obese men.
Type de document :
Article dans une revue
Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2010, 95 (12), pp.5449-53. 〈10.1210/jc.2010-0776〉
Liste complète des métadonnées

Littérature citée [24 références]  Voir  Masquer  Télécharger
http://www.hal.inserm.fr/inserm-00613591
Contributeur : Marie Francoise Simon <>
Soumis le : jeudi 1 décembre 2011 - 10:59:01
Dernière modification le : jeudi 13 septembre 2018 - 10:26:05
Document(s) archivé(s) le : vendredi 2 mars 2012 - 02:20:34

Fichier

5449.full.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

UNIV-TLSE3

Citation

Johan Jocken, Cedric Moro, Gijs Goossens, Dominique Hansen, Aline Mairal, et al.. Skeletal muscle lipase content and activity in obesity and type 2 diabetes.. Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2010, 95 (12), pp.5449-53. 〈10.1210/jc.2010-0776〉. 〈inserm-00613591〉

Exporter

BibTeX TEI DC DCterms EndNote

Partager

Métriques

Consultations de la notice

292

Téléchargements de fichiers

397

Contact

support.ccsd.cnrs.fr
hal.support@ccsd.cnrs.fr
Logo CCSD