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Modulated contact frequencies at gene-rich loci support a statistical helix model for mammalian chromatin organization.

Abstract : ABSTRACT: BACKGROUND: Despite its critical role for mammalian gene regulation, the basic structural landscape of chromatin in living cells remains largely unknown within chromosomal territories below the megabase scale. RESULTS: Here, using the 3C-qPCR method, we investigate contact frequencies at high resolution within the interphase chromatin at several mouse loci. We find that, at several gene-rich loci, contact frequencies undergo a periodical modulation (every 90-100 kb) that affects chromatin dynamics over large genomic distances (few hundred kb). Interestingly, this modulation appears to be conserved in human cells and bioinformatic analyses of locus-specific, long-range cis-interactions suggest that it may underlie the dynamics of a significant number of gene-rich domains in mammals, thus contributing to genome evolution. Finally, using an original model derived from polymer physics, we show that this modulation can be understood as a fundamental helix shape that chromatin tends to adopt in gene-rich domains when no significant locus-specific interaction takes place. CONCLUSIONS: Altogether, our work unveils a fundamental aspect of chromatin dynamics in mammals and contributes to a better understanding of genome organization within chromosomal territories.
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https://www.hal.inserm.fr/inserm-00605349
Contributor : Ed. Bmc <>
Submitted on : Friday, July 1, 2011 - 12:04:32 PM
Last modification on : Monday, December 2, 2019 - 9:48:01 AM
Long-term archiving on: : Monday, November 12, 2012 - 9:52:26 AM

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Franck Court, Julie Miro, Caroline Braem, Marie-Noëlle Lelay-Taha, Audrey Brisebarre, et al.. Modulated contact frequencies at gene-rich loci support a statistical helix model for mammalian chromatin organization.. Genome Biology, BioMed Central, 2011, 12 (5), pp.R42. ⟨10.1186/gb-2011-12-5-r42⟩. ⟨inserm-00605349⟩

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