A regulatory domain is required for Foxn4 activity during retinogenesis.

Elise Lelièvre 1, 2 Bérénice Benayoun 3 Laurence Mahieu 2 Jérôme Roger 2 José-Alain Sahel 2 Florian Sennlaub 1 Reiner Veitia 3 Olivier Goureau 2 Xavier Guillonneau 1, *
* Auteur correspondant
1 CRC (UMR_S 872) - Centre de Recherche des Cordeliers
2 Institut de la Vision
3 IJM - Institut Jacques Monod
Abstract : Foxn4, a member of the N-family forkhead transcription factors, controls fate decision in mouse retina and spinal cord as well as in zebrafish heart. Analysis of Foxn4 amino acid sequence revealed the presence of a region homologous to the activation domain of its close relative Foxn1 in between C-terminal amino acids 402 and 455 of Foxn4 protein. The requirement of Foxn4 putative activation domain remains to be elucidated. Using a gain-of function approach in rat and chick retinal explants, we report that deletion of Foxn4 putative activation domain results in a complete loss of its activity during retinogenesis. Target promoter transcription assay indicates that this domain is critical for Foxn4 transcriptional regulatory properties in vitro. Accordingly, in chick retinal explants, this domain is required for proper regulation of target retinogenic factors expression by Foxn4. Thus, our study demonstrates that the domain between amino acids 402 and 455 is necessary for Foxn4 transcriptional activity both in vitro and in the retina.
Keywords : Foxn4 Foxn1 Forkhead retinogenesis activation domain
Type de document :
Article dans une revue
Journal of Molecular Neuroscience, Humana Press, 2012, 46 (2), pp.315-23. 〈10.1007/s12031-011-9585-4〉
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Contributeur : Xavier Guillonneau <>
Soumis le : jeudi 30 juin 2011 - 09:08:21
Dernière modification le : vendredi 31 août 2018 - 09:13:20
Document(s) archivé(s) le : samedi 1 octobre 2011 - 02:21:33

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Elise Lelièvre, Bérénice Benayoun, Laurence Mahieu, Jérôme Roger, José-Alain Sahel, et al.. A regulatory domain is required for Foxn4 activity during retinogenesis.. Journal of Molecular Neuroscience, Humana Press, 2012, 46 (2), pp.315-23. 〈10.1007/s12031-011-9585-4〉. 〈inserm-00604900〉

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