Skip to Main content Skip to Navigation
Journal articles

A regulatory domain is required for Foxn4 activity during retinogenesis.

Abstract : Foxn4, a member of the N-family forkhead transcription factors, controls fate decision in mouse retina and spinal cord as well as in zebrafish heart. Analysis of Foxn4 amino acid sequence revealed the presence of a region homologous to the activation domain of its close relative Foxn1 in between C-terminal amino acids 402 and 455 of Foxn4 protein. The requirement of Foxn4 putative activation domain remains to be elucidated. Using a gain-of function approach in rat and chick retinal explants, we report that deletion of Foxn4 putative activation domain results in a complete loss of its activity during retinogenesis. Target promoter transcription assay indicates that this domain is critical for Foxn4 transcriptional regulatory properties in vitro. Accordingly, in chick retinal explants, this domain is required for proper regulation of target retinogenic factors expression by Foxn4. Thus, our study demonstrates that the domain between amino acids 402 and 455 is necessary for Foxn4 transcriptional activity both in vitro and in the retina.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00604900
Contributor : Xavier Guillonneau <>
Submitted on : Thursday, June 30, 2011 - 9:08:21 AM
Last modification on : Saturday, April 11, 2020 - 2:12:21 AM
Long-term archiving on: : Saturday, October 1, 2011 - 2:21:33 AM

Files

JOMN-HAL.pdf
Files produced by the author(s)

Identifiers

Citation

Elise Lelièvre, Bérénice Benayoun, Laurence Mahieu, Jérôme Roger, José-Alain Sahel, et al.. A regulatory domain is required for Foxn4 activity during retinogenesis.. Journal of Molecular Neuroscience, Humana Press, 2012, 46 (2), pp.315-23. ⟨10.1007/s12031-011-9585-4⟩. ⟨inserm-00604900⟩

Share

Metrics

Record views

1082

Files downloads

1441