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Journal articles
Dual-color fluorescence lifetime correlation spectroscopy to quantify protein-protein interactions in live cell.
Abstract : Dual-color fluorescence correlation spectroscopy is an interesting method to quantify protein interaction in living cells. But, when performing these experiments, one must compensate for a known spectral bleed through artifact that corrupts cross-correlation data. In this article, problems with crosstalk were overcome with an approach based on fluorescence lifetime correlation spectroscopy (FLCS). We show that FLCS applied to dual-color EGFP and mCherry cross-correlation allows the determination of protein-protein interactions in living cells without the need of spectral bleed through calibration. The methodology was validated by using EGFP-mCherry tandem in comparison with coexpressed EGFP and mCherry in live cell. The dual-color FLCS experimental procedure where the different laser intensities do not have to be controlled during experiment is really very helpful to study quantitatively protein interactions in live sample.
Cited literature [28 references]
https://www.hal.inserm.fr/inserm-00604664
Contributor : Hervé de Villemeur Connect in order to contact the contributor
Submitted on : Thursday, June 30, 2011 - 12:01:10 PM
Last modification on : Thursday, January 14, 2021 - 11:19:50 AM
Long-term archiving on: : Monday, December 5, 2016 - 12:54:33 AM
Contributor : Hervé de Villemeur Connect in order to contact the contributor
Submitted on : Thursday, June 30, 2011 - 12:01:10 PM
Last modification on : Thursday, January 14, 2021 - 11:19:50 AM
Long-term archiving on: : Monday, December 5, 2016 - 12:54:33 AM
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Padilla-Parra_MRT-1.pdf
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