Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC. - Archive ouverte HAL Access content directly
Journal Articles Journal of Experimental and Clinical Cancer Research Year : 2011

Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC.

(1, 2) , (1, 2) , (2, 3) , (4)
1
2
3
4

Abstract

ABSTRACT: BACKGROUND: Epidermal Growth Factor Receptor (EGFR) mutations, especially in-frame deletions in exon 19 (delta LRE) and a point mutation in exon 21 (L858R) predict gefitinib sensitivity in patients with non-small cell lung cancer. Several methods are currently described for their detection but the gold standard for tissue samples remains direct DNA sequencing, which requires samples containing at least 50% of tumor cells. METHODS: We designed a pyrosequencing assay based on nested PCR for the characterization of theses mutations on formalin-fixed and paraffin-embedded tumor tissue. RESULTS: This method is highly specific and permits precise characterization of all the exon 19 deletions. Its sensitivity is higher than that of "BigDye terminator" sequencing and enabled detection of 3 additional mutations in the 58 NSCLC tested. The concordance between the two methods was very good (97.4%). In the prospective analysis of 213 samples, 7 (3.3%) samples were not analyzed and EGFR mutations were detected in 18 (8.7%) patients. However, we observed a deficit of mutation detection when the samples were very poor in tumor cells. CONCLUSIONS: pyrosequencing is then a highly accurate method for detecting delta LRE and L858R EGFR mutations in patients with NSCLC when the samples contain at least 20% of tumor cells.
Fichier principal
Vignette du fichier
1756-9966-30-57.pdf (281.97 Ko) Télécharger le fichier
Vignette du fichier
1756-9966-30-57.xml (53.02 Ko) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Format : Other
Loading...

Dates and versions

inserm-00602408 , version 1 (22-06-2011)

Identifiers

Cite

Sandrine Dufort, Marie-Jeanne Richard, Sylvie Lantuejoul, Florence de Fraipont. Pyrosequencing, a method approved to detect the two major EGFR mutations for anti EGFR therapy in NSCLC.. Journal of Experimental and Clinical Cancer Research, 2011, 30 (1), pp.57. ⟨10.1186/1756-9966-30-57⟩. ⟨inserm-00602408⟩

Collections

INSERM UGA U823
108 View
322 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More