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Towards targeted mutagenesis and gene replacement in plants.

Abstract : Advances in the development of biotechnological tools for plant gene disruption and repair have lagged behind the rapid progress made in whole-genome sequencing of many model and crop plant species. Plant DNA-repair machinery predominantly uses non-homologous end-joining (NHEJ), making the homologous recombination (HR)-based methods, which have proved fruitful for gene targeting in non-plant systems, unsuitable for use in plant systems. Two recent reports describe successful targeted mutagenesis and gene targeting in Arabidopsis by either harnessing the plant NHEJ machinery using site-specific induction of double-strand breaks (DSBs), or by activation of a HR pathway through overexpression of a yeast DNA recombination gene in transgenic plants. These reports provide a foundation from which new technologies for site-specific genome alterations in plant species can be developed.
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Contributor : Charles White <>
Submitted on : Wednesday, May 25, 2011 - 11:20:20 PM
Last modification on : Tuesday, April 20, 2021 - 11:22:02 AM




Tzvi Tzfira, Charles White. Towards targeted mutagenesis and gene replacement in plants.. Trends in Biotechnology, Elsevier, 2005, 23 (12), pp.567-9. ⟨10.1016/j.tibtech.2005.10.002⟩. ⟨inserm-00595923⟩