Skip to Main content Skip to Navigation
Journal articles

Roles of the AtErcc1 protein in recombination.

Abstract : Summary Atercc1, the recently characterized Arabidopsis homologue of the Ercc1 (Rad10) protein, is a key component of nucleotide excision repair as part of a structure-specific endonuclease which cleaves 5' to UV photoproducts in DNA. This endonuclease also acts in removing overhanging non-homologous DNA 'tails' in synapsed recombination intermediates. We have previously demonstrated this recombination function of the Arabidopsis thaliana Xpf homologue, AtRad1p, and show here that recombination between plasmid DNA substrates containing non-homologous tails is specifically reduced 12-fold in atercc1 mutant plants compared with the wild type. Furthermore, using chromosomal tandem-repeat recombination substrates, we show that AtErcc1p is required for bleomycin induction of mitotic recombination in the chromosomal context. This work thus confirms both the specific and general recombination roles of the Atercc1 protein in recombination in Arabidopsis.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00595817
Contributor : Charles White <>
Submitted on : Wednesday, May 25, 2011 - 4:03:29 PM
Last modification on : Monday, November 16, 2020 - 9:08:03 AM

Links full text

Identifiers

Collections

Citation

Sandra Dubest, Maria Gallego, Charles White. Roles of the AtErcc1 protein in recombination.. Plant Journal, Wiley, 2004, 39 (3), pp.334-42. ⟨10.1111/j.1365-313X.2004.02136.x⟩. ⟨inserm-00595817⟩

Share

Metrics

Record views

190