Skip to Main content Skip to Navigation
Journal articles

Two roles for Rad50 in telomere maintenance.

Abstract : We describe two roles for the Rad50 protein in telomere maintenance and the protection of chromosome ends. Using fluorescence in situ hybridisation (FISH) and fibre-FISH analyses, we show that absence of AtRad50 protein leads to rapid shortening of a subpopulation of chromosome ends and subsequently chromosome-end fusions lacking telomeric repeats. In the absence of telomerase, mutation of atrad50 has a synergistic effect on the number of chromosome end fusions. Surprisingly, this 'deprotection' of the shortened telomeres does not result in increased exonucleolytic degradation, but in a higher proportion of anaphase bridges containing telomeric repeats in atrad50/tert plants, compared to tert mutant plants. Absence of AtRad50 thus facilitates the action of recombination on these shortened telomeres. We propose that this protective role of Rad50 protein on shortened telomeres results from its action in constraining recombination to sister chromatids and thus avoiding end-to-end interactions.
Document type :
Journal articles
Complete list of metadatas

https://www.hal.inserm.fr/inserm-00595800
Contributor : Charles White <>
Submitted on : Wednesday, May 25, 2011 - 3:51:38 PM
Last modification on : Wednesday, March 4, 2020 - 11:26:39 AM

Links full text

Identifiers

Collections

Citation

Jean-Baptiste Vannier, Annie Depeiges, Charles White, Maria Eugenia Gallego. Two roles for Rad50 in telomere maintenance.. EMBO Journal, EMBO Press, 2006, 25 (19), pp.4577-85. ⟨10.1038/sj.emboj.7601345⟩. ⟨inserm-00595800⟩

Share

Metrics