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Proximity ligation in situ assay for monitoring the global DNA methylation in cells.

Abstract : ABSTRACT: BACKGROUND: DNA methylation has a central role in the epigenetic control of mammalian gene expression, and is required for X inactivation, genomics imprinting and silencing of retrotransposons and repetitive sequences. Thus, several technologies have been developed to measure the degree of DNA methylation. RESULTS: We here present the development of the detection of protein-protein interactions via the adaptation of the proximity ligation in situ technology to evaluate the DNA methylation status in cells since the quantification of Dnmt1/PCNA interaction in cells reflects the degree of DNA methylation. CONCLUSION: This method being directly realizable on cells, it appears that it could suggest a wide range of applications in basic research and drug development. More particularly, this method is specially adapted for the investigations realized from a weak quantity of biologic materiel such as stem cells or primary cultured tumor cells for examples.
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Submitted on : Wednesday, April 20, 2011 - 1:07:55 PM
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Eric Hervouet, Philippe Hulin, François Vallette, Pierre-François Cartron. Proximity ligation in situ assay for monitoring the global DNA methylation in cells.. BMC Biotechnology, BioMed Central, 2011, 11 (1), pp.31. ⟨10.1186/1472-6750-11-31⟩. ⟨inserm-00587414⟩



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