The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.

Eva Trost; Lisa Ott; Jessica Schneider; Jasmin Schröder; Sebastian Jaenicke; Alexander Goesmann; Peter Husemann; Jens Stoye; Fernanda Dorella; Flavia Rocha; Siomar De Castro Soares; Vívian D'Afonseca; Anderson Miyoshi; Jeronimo Ruiz; Artur Silva; Vasco Azevedo; Andreas Burkovski; Nicole Guiso; Olivier Join-Lambert; Samer Kayal; Andreas Tauch

doi:10.1186/1471-2164-11-728

The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.

Eva Trost ^{1, 2} Lisa Ott ³ Jessica Schneider ^{1, 2, 4} Jasmin Schröder ¹ Sebastian Jaenicke ⁴ Alexander Goesmann ⁴ Peter Husemann ⁵ Jens Stoye ⁵ Fernanda Dorella ⁶ Flavia Rocha ⁶ Siomar De Castro Soares ⁶ Vívian D'Afonseca ⁶ Anderson Miyoshi ⁶ Jeronimo Ruiz ⁷ Artur Silva ⁸ Vasco Azevedo ⁶ Andreas Burkovski ³ Nicole Guiso ⁹ Olivier Join-Lambert ¹⁰ Samer Kayal ¹¹ Andreas Tauch ^{1, *}

* Auteur correspondant

1 Institut für Genomforschung und Systembiologie

2 CLIB Graduate Cluster Industrial Biotechnology

3 Lehrstuhl für Mikrobiologie

4 Bioinformatics Resource Facility

5 AG Genominformatik

6 Laboratório de Genética Celular e Molecular

7 Cellular and Molecular Parasitology Laboratory

8 Instituto de Ciências Biológicas

9 Prévention et Thérapie Moléculaires des Maladies Infectieuses Humaines

10 Service de Microbiologie

11 CHU Pontchaillou [Rennes]

Abstract : BACKGROUND: Corynebacterium pseudotuberculosis is generally regarded as an important animal pathogen that rarely infects humans. Clinical strains are occasionally recovered from human cases of lymphadenitis, such as C. pseudotuberculosis FRC41 that was isolated from the inguinal lymph node of a 12-year-old girl with necrotizing lymphadenitis. To detect potential virulence factors and corresponding gene-regulatory networks in this human isolate, the genome sequence of C. pseudotuberculosis FCR41 was determined by pyrosequencing and functionally annotated. RESULTS: Sequencing and assembly of the C. pseudotuberculosis FRC41 genome yielded a circular chromosome with a size of 2,337,913 bp and a mean G+C content of 52.2%. Specific gene sets associated with iron and zinc homeostasis were detected among the 2,110 predicted protein-coding regions and integrated into a gene-regulatory network that is linked with both the central metabolism and the oxidative stress response of FRC41. Two gene clusters encode proteins involved in the sortase-mediated polymerization of adhesive pili that can probably mediate the adherence to host tissue to facilitate additional ligand-receptor interactions and the delivery of virulence factors. The prominent virulence factors phospholipase D (Pld) and corynebacterial protease CP40 are encoded in the genome of this human isolate. The genome annotation revealed additional serine proteases, neuraminidase H, nitric oxide reductase, an invasion-associated protein, and acyl-CoA carboxylase subunits involved in mycolic acid biosynthesis as potential virulence factors. The cAMP-sensing transcription regulator GlxR plays a key role in controlling the expression of several genes contributing to virulence. CONCLUSION: The functional data deduced from the genome sequencing and the extended knowledge of virulence factors indicate that the human isolate C. pseudotuberculosis FRC41 is equipped with a distinct gene set promoting its survival under unfavorable environmental conditions encountered in the mammalian host.

Type de document :

Article dans une revue

BMC Genomics, BioMed Central, 2010, 11 (1), pp.728. 〈10.1186/1471-2164-11-728〉

Domaine :

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Génomique, Transcriptomique et Protéomique [q-bio.GN]

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The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.

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The complete genome sequence of Corynebacterium pseudotuberculosis FRC41 isolated from a 12-year-old girl with necrotizing lymphadenitis reveals insights into gene-regulatory networks contributing to virulence.

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