High-resolution simultaneous voltage and Ca2+ imaging. - Archive ouverte HAL Access content directly
Journal Articles The Journal of Physiology Year : 2011

High-resolution simultaneous voltage and Ca2+ imaging.

(1) , (1) , (2) , (3)
1
2
3

Abstract

Combining voltage and Ca(2+) imaging allows the correlation of electrical and chemical activity at sub-cellular level. Here we describe a novel apparatus designed to obtain simultaneous voltage and Ca(2+) measurements with single-trial resolution from sites as small as a few microns. These measurements can be obtained with negligible optical cross-talk between the two signals and negligible photo-damage of the preparation. The capability of the technique was assessed recording either from individual neurons in brain slices or from networks of cultured neurons. The present achievements open the gate to many novel physiological investigations requiring simultaneous measurement of voltage and Ca(2+) signals.
Fichier principal
Vignette du fichier
Vogt_etal_revised.pdf (591.88 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

inserm-00585501 , version 1 (29-11-2011)

Identifiers

Cite

Kaspar E. Vogt, Stephan Gerharz, Jeremy Graham, Marco Canepari. High-resolution simultaneous voltage and Ca2+ imaging.. The Journal of Physiology, 2011, 589 (Pt 3), pp.489-94. ⟨10.1113/jphysiol.2010.200220⟩. ⟨inserm-00585501⟩

Collections

INSERM UGA U836
189 View
245 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More