Skip to Main content Skip to Navigation
Journal articles

Fabrication of Bio-Functionalised Polypyrrole Nanoarrays for Bio-Molecular Recognition

Abstract : The present study demonstrates that nanosphere lithography and electro-polymerization can be successfully combined to produce bioactive protein nanoarrays. In particular, we describe a method to produce well-defined nanoarrays of polypyrrole functionalized with biomolecules. The nanoarrayed surfaces were fabricated on gold coated surface plasmon resonance prisms by first creating silicon oxide or polyethylene oxide nanotemplate using nanosphere lithography. The nanotemplate was subsequently used to grow bio-functionalized polypyrrole nanoarrays by electrocopolymerization. Atomic force microscopy analysis showed that the fabricated surfaces have a well-organized 2D hexagonal geometry with nanoscale dimensions. The biological activity of the bio-functionalized polypyrrole was assessed by surface plasmon resonance detection. The results showed that the immobilized biomolecules within the nanoarrayed polypyrrole films had the necessary bioactivity for successful molecular recognition. Moreover the detection signals normalized to the bioactive area were increased by a factor 5 as compared to non-structured bio-functionalized polypyrrole in the nanoarrayed surfaces using polyethylene oxide.
Complete list of metadatas

Cited literature [28 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00582611
Contributor : Patrice Marche <>
Submitted on : Sunday, October 2, 2011 - 7:00:04 AM
Last modification on : Friday, August 23, 2019 - 3:12:06 PM
Long-term archiving on: : Tuesday, January 3, 2012 - 2:20:07 AM

File

Lisboa_et_al_2011.pdf
Files produced by the author(s)

Identifiers

  • HAL Id : inserm-00582611, version 1

Collections

U823 | UGA

Citation

Patrícia Lisboa, Marie-Bernadette Villiers, Carine Brakha, Patrice Marche, Andrea Valsesia, et al.. Fabrication of Bio-Functionalised Polypyrrole Nanoarrays for Bio-Molecular Recognition. Micro and Nanosystems, Bentham Science Publishers, 2011, 3, pp.83-89. ⟨inserm-00582611⟩

Share

Metrics

Record views

468

Files downloads

443