Skip to Main content Skip to Navigation
Journal articles

CDK11(p58) is required for centriole duplication and Plk4 recruitment to mitotic centrosomes.

Abstract : BACKGROUND: CDK11(p58) is a mitotic protein kinase, which has been shown to be required for different mitotic events such as centrosome maturation, chromatid cohesion and cytokinesis. METHODOLOGY/PRINCIPAL FINDINGS: In addition to these previously described roles, our study shows that CDK11(p58) inhibition induces a failure in the centriole duplication process in different human cell lines. We propose that this effect is mediated by the defective centrosomal recruitment of proteins at the onset of mitosis. Indeed, Plk4 protein kinase and the centrosomal protein Cep192, which are key components of the centriole duplication machinery, showed reduced levels at centrosomes of mitotic CDK11-depleted cells. CDK11(p58), which accumulates only in the vicinity of mitotic centrosomes, directly interacts with the centriole-associated protein kinase Plk4 that regulates centriole number in cells. In addition, we show that centriole from CDK11 defective cells are not able to be over duplicated following Plk4 overexpression. CONCLUSION/SIGNIFICANCE: We thus propose that CDK11 is required for centriole duplication by two non-mutually-exclusive mechanisms. On one hand, the observed duplication defect could be caused indirectly by a failure of the centrosome to fully maturate during mitosis. On the other hand, CDK11(p58) could also directly regulate key centriole components such as Plk4 during mitosis to trigger essential mitotic centriole modifications, required for centriole duplication during subsequent interphase.
Document type :
Journal articles
Complete list of metadatas

Cited literature [36 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00561837
Contributor : Hervé de Villemeur <>
Submitted on : Wednesday, February 2, 2011 - 9:56:19 AM
Last modification on : Thursday, December 12, 2019 - 12:22:02 PM
Long-term archiving on: : Tuesday, May 3, 2011 - 2:47:55 AM

File

journal.pone.0014600.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Nathalie Franck, Emilie Montembault, Pierre Romé, Aude Pascal, Jean-Yves Cremet, et al.. CDK11(p58) is required for centriole duplication and Plk4 recruitment to mitotic centrosomes.. PLoS ONE, Public Library of Science, 2011, 6 (1), pp.e14600. ⟨10.1371/journal.pone.0014600⟩. ⟨inserm-00561837⟩

Share

Metrics

Record views

379

Files downloads

717