Evaluation of alginate microspheres for mesenchymal stem cells engraftment on solid organ.

Abstract : Mesenchymal stem cells (MSCs) may be used as a cell source for cell therapy of solid organ due to their differentiation potential and paracrine effect. Nevertheless, optimisation of MSCs based therapy needs to develop alternative strategies to improve cell administration and efficiency. One option is the use of alginate microencapsulation which presents an excellent biocompatibility and an in vivo stability. As MSCs are hypoimmunogenic, it was conceivable to produce microparticles with (alginate-poly(L-lysine)-alginate (APA) microcapsules) or without (alginate microspheres) a surrounding protective membrane. Therefore, the aim of this study was to determine the most suitable microparticles to encapsulate MSCs for engraftment on solid organ. First, we compared the two types of microparticles with 4 x 10 MSCs/ ml of alginate. Results showed that each microparticle has distinct morphology and mechanical resistance but both remained stable over time. However, as MSCs exhibited a better viability in microspheres than in microcapsules, the study was pursued with microspheres. We demonstrated that viable MSCs were still able to produce the paracrine factor b-FGF and did not present any chondrogenic or osteogenic differentiation, processes sometimes reported with the use of polymers. We then proved that microspheres could be implanted under the renal capsule without degradation with time or inducing impairment of renal function. In conclusion, these microspheres behave as an implantable scaffold whose biological and functional properties could be adapted to fit with clinical applications.
Type de document :
Article dans une revue
Cell Transplantation, Cognizant Communication Corporation, 2010, epub ahead of print. 〈10.3727/096368910X514297〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00550872
Contributeur : Marie Francoise Simon <>
Soumis le : vendredi 31 décembre 2010 - 15:32:57
Dernière modification le : jeudi 13 septembre 2018 - 10:26:05

Lien texte intégral

Identifiants

Collections

Citation

Elodie Trouche, Sophie Girod-Fullana, Céline Mias, Caroline Ceccaldi, Florence Tortosa, et al.. Evaluation of alginate microspheres for mesenchymal stem cells engraftment on solid organ.. Cell Transplantation, Cognizant Communication Corporation, 2010, epub ahead of print. 〈10.3727/096368910X514297〉. 〈inserm-00550872〉

Partager

Métriques

Consultations de la notice

130