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The G0/G1 switch gene 2 is a novel PPAR target gene.

Abstract : PPARs (peroxisome-proliferator-activated receptors) alpha, beta/delta and gamma are a group of transcription factors that are involved in numerous processes, including lipid metabolism and adipogenesis. By comparing liver mRNAs of wild-type and PPARalpha-null mice using microarrays, a novel putative target gene of PPARalpha, G0S2 (G0/G1 switch gene 2), was identified. Hepatic expression of G0S2 was up-regulated by fasting and by the PPARalpha agonist Wy14643 in a PPARalpha-dependent manner. Surprisingly, the G0S2 mRNA level was highest in brown and white adipose tissue and was greatly up-regulated during mouse 3T3-L1 and human SGBS (Simpson-Golabi-Behmel syndrome) adipogenesis. Transactivation, gel shift and chromatin immunoprecipitation assays indicated that G0S2 is a direct PPARgamma and probable PPARalpha target gene with a functional PPRE (PPAR-responsive element) in its promoter. Up-regulation of G0S2 mRNA seemed to be specific for adipogenesis, and was not observed during osteogenesis or myogenesis. In 3T3-L1 fibroblasts, expression of G0S2 was associated with growth arrest, which is required for 3T3-L1 adipogenesis. Together, these data indicate that G0S2 is a novel target gene of PPARs that may be involved in adipocyte differentiation.
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Contributor : Stéphane Mandard <>
Submitted on : Monday, December 13, 2010 - 4:58:42 PM
Last modification on : Saturday, October 17, 2020 - 7:16:03 PM
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Fokko Zandbergen, Stéphane Mandard, Pascal Escher, Nguan Soon Tan, David Patsouris, et al.. The G0/G1 switch gene 2 is a novel PPAR target gene.. Biochemical Journal, Portland Press, 2005, 392 (Pt 2), pp.313-24. ⟨10.1042/BJ20050636⟩. ⟨inserm-00546129⟩



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