 |
Journal articles
Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina.
Abstract : Acidic and basic fibroblast growth factors (FGFs) influence cell division and differentiation in retina cells. Their effects are thought to be mainly mediated through stimulation of a specific membrane receptor and subsequent generation of an intracellular signal pathway. In this study, we purified a FGF receptor of 130 kDa from bovine neural retina using wheat germ agglutinin affinity chromatography followed by FGF-affinity chromatography. The isolated receptor showed ligand binding activity with dissociation constants of 0.8 nM and 2 nM for aFGF and bFGF, respectively. Furthermore, binding of aFGF and bFGF to purified receptor resulted in self-phosphorylation, demonstrating that the isolated receptor had an unaltered intrinsic kinase activity.
https://www.hal.inserm.fr/inserm-00537599
Contributor : Alicia Torriglia <>
Submitted on : Thursday, November 18, 2010 - 5:17:03 PM
Last modification on : Tuesday, July 3, 2018 - 9:34:10 AM
Contributor : Alicia Torriglia <>
Submitted on : Thursday, November 18, 2010 - 5:17:03 PM
Last modification on : Tuesday, July 3, 2018 - 9:34:10 AM