Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina. - Archive ouverte HAL Access content directly
Journal Articles Biochimica et Biophysica Acta - Molecular Cell Research Year : 1992

Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina.

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1
Alicia Torriglia
  • Function : Author
Pierre R. Blanquet
  • Function : Author

Abstract

Acidic and basic fibroblast growth factors (FGFs) influence cell division and differentiation in retina cells. Their effects are thought to be mainly mediated through stimulation of a specific membrane receptor and subsequent generation of an intracellular signal pathway. In this study, we purified a FGF receptor of 130 kDa from bovine neural retina using wheat germ agglutinin affinity chromatography followed by FGF-affinity chromatography. The isolated receptor showed ligand binding activity with dissociation constants of 0.8 nM and 2 nM for aFGF and bFGF, respectively. Furthermore, binding of aFGF and bFGF to purified receptor resulted in self-phosphorylation, demonstrating that the isolated receptor had an unaltered intrinsic kinase activity.
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Dates and versions

inserm-00537599 , version 1 (18-11-2010)

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  • HAL Id : inserm-00537599 , version 1
  • PUBMED : 1384713

Cite

Alicia Torriglia, Pierre R. Blanquet. Purification of an active receptor for acidic and basic fibroblast growth factor from bovine retina.. Biochimica et Biophysica Acta - Molecular Cell Research, 1992, 1137 (2), pp.215-24. ⟨inserm-00537599⟩

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