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β1A integrin is a master regulator of invadosome organization and function.: β1 integrin control of invadosomes

Abstract : Invadosomes are adhesion structures involved in tissue invasion that are characterized by an intense actin polymerization-depolymerization associated with β1 and β3 integrins and coupled to extracellular matrix (ECM) degradation activity. We induced the formation of invadosomes by expressing the constitutive active form of Src, SrcYF, in different cell types. Use of ECM surfaces micropatterned at the subcellular scale clearly showed that in mesenchymal cells, integrin signaling controls invadosome activity. Using β1⁻/⁻ or β3⁻/⁻ cells, it seemed that β1A but not β3 integrins are essential for initiation of invadosome formation. Protein kinase C activity was shown to regulate autoassembly of invadosomes into a ring-like metastructure (rosette), probably by phosphorylation of Ser785 on the β1A tail. Moreover, our study clearly showed that β1A links actin dynamics and ECM degradation in invadosomes. Finally, a new strategy based on fusion of the photosensitizer KillerRed to the β1A cytoplasmic domain allowed specific and immediate loss of function of β1A, resulting in disorganization and disassembly of invadosomes and formation of focal adhesions.
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Submitted on : Tuesday, October 26, 2010 - 11:29:51 AM
Last modification on : Friday, November 6, 2020 - 4:15:04 AM
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Olivier Destaing, Emmanuelle Planus, Daniel Bouvard, Christiane Oddou, Cedric Badowski, et al.. β1A integrin is a master regulator of invadosome organization and function.: β1 integrin control of invadosomes. Molecular Biology of the Cell, American Society for Cell Biology, 2010, 21 (23), pp.4108-19. ⟨10.1091/mbc.E10-07-0580⟩. ⟨inserm-00529654⟩

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