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The role of fluctuations and stress on the effective viscosity of cell aggregates.

Abstract : Cell aggregates are a tool for in vitro studies of morphogenesis, cancer invasion, and tissue engineering. They respond to mechanical forces as a complex rather than simple liquid. To change an aggregate's shape, cells have to overcome energy barriers. If cell shape fluctuations are active enough, the aggregate spontaneously relaxes stresses ("fluctuation-induced flow"). If not, changing the aggregate's shape requires a sufficiently large applied stress ("stress-induced flow"). To capture this distinction, we develop a mechanical model of aggregates based on their cellular structure. At stress lower than a characteristic stress tau*, the aggregate as a whole flows with an apparent viscosity eta*, and at higher stress it is a shear-thinning fluid. An increasing cell-cell tension results in a higher eta* (and thus a slower stress relaxation time t(c)). Our constitutive equation fits experiments of aggregate shape relaxation after compression or decompression in which irreversibility can be measured; we find t(c) of the order of 5 h for F9 cell lines. Predictions also match numerical simulations of cell geometry and fluctuations. We discuss the deviations from liquid behavior, the possible overestimation of surface tension in parallel-plate compression measurements, and the role of measurement duration.
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https://www.hal.inserm.fr/inserm-00524596
Contributor : Jean-Paul Issartel <>
Submitted on : Friday, October 8, 2010 - 11:46:16 AM
Last modification on : Thursday, October 15, 2020 - 9:00:03 AM
Long-term archiving on: : Friday, December 2, 2016 - 6:51:33 AM

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Philippe Marmottant, Abbas Mgharbel, Jos Käfer, Benjamin Audren, Jean-Paul Rieu, et al.. The role of fluctuations and stress on the effective viscosity of cell aggregates.. Proceedings of the National Academy of Sciences of the United States of America , National Academy of Sciences, 2009, 106 (41), pp.17271-5. ⟨10.1073/pnas.0902085106⟩. ⟨inserm-00524596⟩

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