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A novel in vitro preparation: the intact hippocampal formation.

Abstract : The intact hippocampal formation (IHF) of neonatal or young rats can be kept alive for an extended period in a fully submerged chamber with excellent morphological preservation. Field or patch-clamp recordings, intracellular Ca2+ measurements, and 3-D reconstruction of biocytin-filled neurons can be performed routinely. The generation and propagation of network-driven activities can be studied within the IHF or between connected intact structures such as the septum and the hippocampus or two hippocampi, and the use of a dual chamber enables the application of drugs separately to each structure. This preparation will be useful to study intact neuronal networks in the developing hippocampus in vitro.
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Submitted on : Thursday, September 30, 2010 - 4:44:56 PM
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  • HAL Id : inserm-00522462, version 1
  • PUBMED : 9354321

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Ilgam Khalilov, Monique Esclapez, Igor Medina, Djamila Aggoun, Karri Lamsa, et al.. A novel in vitro preparation: the intact hippocampal formation.. Neuron, Elsevier, 1997, 19 (4), pp.743-9. ⟨inserm-00522462⟩

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