Skip to Main content Skip to Navigation
Journal articles

Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging

Abstract : Chronic exposure to manganese results in neurological symptoms referred to as manganism and is identified as a risk factor for Parkinson's disease. In vitro, manganese induces cell death in the dopaminergic cells, but the mechanisms of manganese cytotoxicity are still unexplained. In particular, the subcellular distribution of manganese and its interaction with other trace elements needed to be assessed. Applying synchrotron X-ray fluorescence nanoimaging, we found that manganese was located within the Golgi apparatus of PC12 dopaminergic cells at physiologic concentrations. At increasing concentrations, manganese accumulates within the Golgi apparatus until cytotoxic concentrations are reached resulting in a higher cytoplasmic content probably after the Golgi apparatus storage capacity is exceeded. Cell exposure to manganese and brefeldin A, a molecule known to specifically cause the collapse of the Golgi apparatus, results in the striking intracellular redistribution of manganese, which accumulates in the cytoplasm and the nucleus. These results indicate that the Golai apparatus plays an important role in the cellular detoxification of manganese. In addition manganese exposure induces a decrease in total iron content, which could contribute to the overall neurotoxicity.
Complete list of metadatas

Cited literature [46 references]  Display  Hide  Download

https://www.hal.inserm.fr/inserm-00522281
Contributor : Raphael Serduc <>
Submitted on : Thursday, September 30, 2010 - 12:37:32 PM
Last modification on : Wednesday, July 15, 2020 - 2:34:10 PM
Long-term archiving on: : Friday, December 2, 2016 - 7:30:46 AM

File

 Restricted access
To satisfy the distribution rights of the publisher, the document is embargoed until : jamais

Please log in to resquest access to the document

Identifiers

Collections

CNRS | U836 | UGA

Citation

Asuncion Carmona, Guillaume Devès, Stéphane Roudeau, Peter Cloetens, Sylvain Bohic, et al.. Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging. ACS Chemical Neuroscience, American Chemical Society (ACS), 2010, 1 (3), pp.194-203. ⟨10.1021/cn900021z⟩. ⟨inserm-00522281⟩

Share

Metrics

Record views

218