Skip to Main content Skip to Navigation
Journal articles

Getting closer to the patient: upgrade of hepatitis C virus infection in primary human hepatocytes.

Abstract : Hepatitis C virus (HCV) remains a major public health problem, affecting approximately 130 million people worldwide. HCV infection can lead to cirrhosis, hepatocellular carcinoma, and end-stage liver disease, as well as extrahepatic complications such as cryoglobulinemia and lymphoma. Preventative and therapeutic options are severely limited; there is no HCV vaccine available, and nonspecific, IFN-based treatments are frequently ineffective. Development of targeted antivirals has been hampered by the lack of robust HCV cell culture systems that reliably predict human responses. Here, we show the entire HCV life cycle recapitulated in micropatterned cocultures (MPCCs) of primary human hepatocytes and supportive stroma in a multiwell format. MPCCs form polarized cell layers expressing all known HCV entry factors and sustain viral replication for several weeks. When coupled with highly sensitive fluorescence- and luminescence-based reporter systems, MPCCs have potential as a high-throughput platform for simultaneous assessment of in vitro efficacy and toxicity profiles of anti-HCV therapeutics.
Complete list of metadata

Cited literature [9 references]  Display  Hide  Download
Contributor : Thomas F. Baumert Connect in order to contact the contributor
Submitted on : Monday, September 13, 2010 - 3:19:02 PM
Last modification on : Thursday, November 26, 2020 - 3:56:04 PM
Long-term archiving on: : Tuesday, December 14, 2010 - 2:54:50 AM


Files produced by the author(s)




Mirjam B. Zeisel, Marine Turek, Thomas F. Baumert. Getting closer to the patient: upgrade of hepatitis C virus infection in primary human hepatocytes.. Journal of Hepatology, Elsevier, 2010, 53 (2), pp.388-9. ⟨10.1016/j.jhep.2010.04.004⟩. ⟨inserm-00517102⟩



Record views


Files downloads